In Vitro Evaluation of Wound Healing, Stemness Potentiation, Antioxidant Activity, and Phytochemical Profile of Cucurbita moschata Duchesne Fruit Pulp Ethanolic Extract.

IF 2.1 Q3 PHARMACOLOGY & PHARMACY Advances in Pharmacological and Pharmaceutical Sciences Pub Date : 2024-10-28 eCollection Date: 2024-01-01 DOI:10.1155/2024/9288481
Preeyaporn Plaimee Phiboonchaiyanan, Saraporn Harikarnpakdee, Thanapat Songsak, Verisa Chowjarean
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Abstract

Wound healing comprises an intricate process to repair damaged tissue. Research on plant extracts with properties to expedite wound healing has been of interest, particularly their ability to enhance the stemness of keratinocyte stem cells. Hence, the present study aims to determine the wound healing and stemness potentiation properties of an ethanolic extract derived from Cucurbita moschata fruit pulp (PKE). Human keratinocytes (HaCaT) and primary skin fibroblast cells were used in this study. The migration of the cells was examined by using a scratch wound healing assay, and spheroid behavior was determined by using a spheroid formation assay. The proteins related to migration and stemness were further measured by using Western blotting to explore the mechanism of action of PKE. The methods used to evaluate PKE's antioxidant properties were 2,2-diphenyl-2-picrylhydrazyl (DPPH) scavenging, ABTS radical scavenging activity, and superoxide anion radical scavenging (SOSA) assays. The phytochemistry of the PKE was investigated using phytochemical screening and high-performance liquid chromatography (HPLC) analysis. The results of this study indicate that nontoxic concentrations of PKE increase the rate of migration and spheroid formation. Mechanistically, PKE increased the expression of the migratory-related protein active FAK (phosphorylated FAK), and the subsequence increased the level of p-AKT. The expression of stem cell marker CD133, upstream protein signaling β-catenin, and self-renewal transcription factor Nanog was increased. The PKE also possessed scavenging properties against DPPH, ABTS, and SOSA. The phytochemistry analyses exhibited the presence of alkaloids, glycosides, xanthones, triterpenes, and steroids. Additionally, bioactive compounds such as ɑ-tocopherol, riboflavin, protocatechuic acid, β-carotene, and luteolin were detected. The presence of these chemicals in PKE may contribute to its antioxidant, stem cell potentiation, and wound-healing effects. The findings could be beneficial in the identification of valuable natural resources that possess the capacity to be used in the process of wound healing through the potentiation of stemness via a readily detectable molecular mechanism.

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对葫芦果肉乙醇提取物的伤口愈合、茎潜能、抗氧化活性和植物化学成分进行体外评估。
伤口愈合是一个修复受损组织的复杂过程。对具有加速伤口愈合特性的植物提取物的研究一直备受关注,尤其是它们增强角质细胞干细胞干性的能力。因此,本研究旨在确定葫芦果肉乙醇提取物(PKE)的伤口愈合和干细胞增效特性。本研究使用了人类角质细胞(HaCaT)和原代皮肤成纤维细胞。通过划痕伤口愈合试验检测了细胞的迁移,并通过球形形成试验确定了细胞的球形行为。此外,还使用 Western 印迹法测定了与迁移和干性相关的蛋白质,以探索 PKE 的作用机制。评估PKE抗氧化特性的方法包括2,2-二苯基-2-苦基肼(DPPH)清除、ABTS自由基清除活性和超氧阴离子自由基清除(SOSA)试验。通过植物化学筛选和高效液相色谱(HPLC)分析,对 PKE 的植物化学成分进行了研究。研究结果表明,无毒浓度的 PKE 可提高迁移率和球形体形成率。从机理上讲,PKE增加了迁移相关蛋白活性FAK(磷酸化FAK)的表达,随后增加了p-AKT的水平。干细胞标志物CD133、上游信号蛋白β-catenin和自我更新转录因子Nanog的表达也有所增加。PKE 还具有清除 DPPH、ABTS 和 SOSA 的特性。植物化学分析显示了生物碱、苷类、黄酮、三萜类和类固醇的存在。此外,还检测到ɑ-生育酚、核黄素、原儿茶酸、β-胡萝卜素和叶黄素等生物活性化合物。PKE中存在的这些化学物质可能有助于其抗氧化、干细胞增效和伤口愈合作用。这些发现有助于发现有价值的自然资源,这些资源具有通过易于检测的分子机制增强干细胞能力的能力,可用于伤口愈合过程。
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来源期刊
CiteScore
4.30
自引率
3.60%
发文量
0
审稿时长
17 weeks
期刊最新文献
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