Dual-Response Functionalized Mitochondrial Fluorescent Probe for a Double Whammy Monitoring of Hypochlorite and Sulfur Dioxide in Heat Shock via Time Scales.

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-11-19 Epub Date: 2024-11-06 DOI:10.1021/acs.analchem.4c05488
Hongshuai Cao, Feifei Yu, Kun Dou, Rui Wang, Yanlong Xing, Xianzhu Luo, Fabiao Yu
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Abstract

Heat shock seriously affects the normal functioning of an organism and can lead to damage and even death in severe cases. To prevent or treat heat shock-related diseases, we require a better understanding of the mechanism of thermocytotoxicity. Here, we designed a functionalized dual-response fluorescent probe (HCy-SO2-HClO) that could individually or simultaneously detect hypochlorous acid (HClO) and sulfur dioxide (SO2) without interfering with each other and achieved the simultaneous tracing of both during the heat shock process for the first time. The introduction of the sulfonate group greatly increased the water solubility of the probe. Furthermore, the probe could effectively accumulate in the mitochondrial region. HCy-SO2-HClO could respond to HClO and SO2 within 10 s and 20 min, respectively, realizing a double whammy detection of both on the time scale. HCy-SO2-HClO exhibited high specificity and sensitivity for HClO and SO2. The highly biocompatible probe HCy-SO2-HClO successfully achieved the detection of endogenous and exogenous SO2 and HClO in living cells and in zebrafish. Moreover, the simultaneous detection of HClO and SO2 in heat shock cells and mouse intestines was realized for the first time. This probe has achieved the detection of dual markers, which enhanced the accuracy and precision of disease detection and could serve as an effective research tool to prevent heat stroke-related diseases.

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双响应功能化线粒体荧光探针通过时间尺度对热休克中的次氯酸盐和二氧化硫进行双重监测
热休克严重影响生物体的正常功能,严重时可导致损伤甚至死亡。为了预防或治疗热休克相关疾病,我们需要更好地了解热细胞毒性的机理。在此,我们设计了一种功能化的双响应荧光探针(HCy-SO2-HClO),可单独或同时检测次氯酸(HClO)和二氧化硫(SO2),且互不干扰,并首次实现了在热休克过程中同时追踪这两种物质。磺酸基团的引入大大提高了探针的水溶性。此外,探针还能有效地积聚在线粒体区域。HCy-SO2-HClO 可分别在 10 秒和 20 分钟内对 HClO 和 SO2 做出反应,实现了在时间尺度上对这两种物质的双重检测。HCy-SO2-HClO 对 HClO 和 SO2 具有很高的特异性和灵敏度。高生物相容性探针 HCy-SO2-HClO 成功实现了活细胞和斑马鱼体内外源性 SO2 和 HClO 的检测。此外,还首次实现了在热休克细胞和小鼠肠道中同时检测 HClO 和 SO2。该探针实现了双重标记物的检测,提高了疾病检测的准确性和精确度,可作为预防中暑相关疾病的有效研究工具。
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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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