Dual-Response Functionalized Mitochondrial Fluorescent Probe for a Double Whammy Monitoring of Hypochlorite and Sulfur Dioxide in Heat Shock via Time Scales.
{"title":"Dual-Response Functionalized Mitochondrial Fluorescent Probe for a Double Whammy Monitoring of Hypochlorite and Sulfur Dioxide in Heat Shock via Time Scales.","authors":"Hongshuai Cao, Feifei Yu, Kun Dou, Rui Wang, Yanlong Xing, Xianzhu Luo, Fabiao Yu","doi":"10.1021/acs.analchem.4c05488","DOIUrl":null,"url":null,"abstract":"<p><p>Heat shock seriously affects the normal functioning of an organism and can lead to damage and even death in severe cases. To prevent or treat heat shock-related diseases, we require a better understanding of the mechanism of thermocytotoxicity. Here, we designed a functionalized dual-response fluorescent probe (<b>HCy-SO<sub>2</sub>-HClO</b>) that could individually or simultaneously detect hypochlorous acid (HClO) and sulfur dioxide (SO<sub>2</sub>) without interfering with each other and achieved the simultaneous tracing of both during the heat shock process for the first time. The introduction of the sulfonate group greatly increased the water solubility of the probe. Furthermore, the probe could effectively accumulate in the mitochondrial region. <b>HCy-SO<sub>2</sub>-HClO</b> could respond to HClO and SO<sub>2</sub> within 10 s and 20 min, respectively, realizing a double whammy detection of both on the time scale. <b>HCy-SO<sub>2</sub>-HClO</b> exhibited high specificity and sensitivity for HClO and SO<sub>2</sub>. The highly biocompatible probe <b>HCy-SO<sub>2</sub>-HClO</b> successfully achieved the detection of endogenous and exogenous SO<sub>2</sub> and HClO in living cells and in zebrafish. Moreover, the simultaneous detection of HClO and SO<sub>2</sub> in heat shock cells and mouse intestines was realized for the first time. This probe has achieved the detection of dual markers, which enhanced the accuracy and precision of disease detection and could serve as an effective research tool to prevent heat stroke-related diseases.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":"18574-18583"},"PeriodicalIF":6.7000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c05488","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/6 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Heat shock seriously affects the normal functioning of an organism and can lead to damage and even death in severe cases. To prevent or treat heat shock-related diseases, we require a better understanding of the mechanism of thermocytotoxicity. Here, we designed a functionalized dual-response fluorescent probe (HCy-SO2-HClO) that could individually or simultaneously detect hypochlorous acid (HClO) and sulfur dioxide (SO2) without interfering with each other and achieved the simultaneous tracing of both during the heat shock process for the first time. The introduction of the sulfonate group greatly increased the water solubility of the probe. Furthermore, the probe could effectively accumulate in the mitochondrial region. HCy-SO2-HClO could respond to HClO and SO2 within 10 s and 20 min, respectively, realizing a double whammy detection of both on the time scale. HCy-SO2-HClO exhibited high specificity and sensitivity for HClO and SO2. The highly biocompatible probe HCy-SO2-HClO successfully achieved the detection of endogenous and exogenous SO2 and HClO in living cells and in zebrafish. Moreover, the simultaneous detection of HClO and SO2 in heat shock cells and mouse intestines was realized for the first time. This probe has achieved the detection of dual markers, which enhanced the accuracy and precision of disease detection and could serve as an effective research tool to prevent heat stroke-related diseases.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.