Associations of Vitamin D With GPX4 and Iron Parameters in Chronic Obstructive Pulmonary Disease Patients: A Case-Control Study.

IF 2.1 4区 医学 Q3 RESPIRATORY SYSTEM Canadian respiratory journal Pub Date : 2024-10-28 eCollection Date: 2024-01-01 DOI:10.1155/2024/4505905
Jun Fei, Ling Liu, Jia-Fei Li, Qiang Zhou, Yu Wei, Ting-Dong Zhou, Lin Fu
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Abstract

Background: Vitamin D deficiency elevates the risk of chronic obstructive pulmonary disease (COPD) patients. Iron parameters elevation and glutathione peroxidase 4 (GPX4) reduction are involved in the process of COPD. The goal is to explore the associations of vitamin D with GPX4 and iron parameters in COPD patients through a case-control study. Methods: COPD patients and control subjects were enrolled. Serum samples and lung tissues were collected. Serum vitamin D and iron levels and pulmonary ferritin and GPX4 expressions were determined. In addition, human pulmonary epithelial cells (BEAS-2B) were incubated with 1,25(OH)2D3 (100 nM), the active form of vitamin D3. Then, vitamin D receptor (VDR) and nuclear factor (erythroid-derived 2)-like 2 (Nrf-2) signaling were detected. Results: In patients with COPD, serum 25-hydroxyvitamin D (25(OH)D) decreased, and iron and ferritin levels in serum and lung tissues increased. Furthermore, pulmonary expression of GPX4 was reduced. Correlative analyzes indicated that lung function was inversely correlated with iron parameters and positively correlated with GPX4. The results showed that serum 25(OH)D deficiency was associated with an elevation in serum iron parameters and a reduction in pulmonary GPX4. In addition, VDR- and Nrf-2-positive lung nuclei were decreased in COPD patients than in control subjects. In patients with COPD, the results indicated a positive relationship between VDR and Nrf-2. Further analysis revealed that Nrf-2-positive nuclei were negatively correlated with iron parameters. In vitro experiments found that 1,25(OH)2D3 treatment activated VDR signaling and elevated the expression of Nrf-2 and GPX4 in BEAS-2B cells. Conclusions: Vitamin D deficiency is positively associated with GPX4 reduction and iron parameters elevation in COPD patients. It is recommended to explore the role of vitamin D supplementation in the progression of COPD.

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慢性阻塞性肺病患者维生素 D 与 GPX4 和铁参数的关系:病例对照研究
背景:维生素 D 缺乏会增加慢性阻塞性肺病(COPD)患者的患病风险。铁参数的升高和谷胱甘肽过氧化物酶 4 (GPX4) 的减少参与了慢性阻塞性肺病的发病过程。本研究旨在通过病例对照研究,探讨 COPD 患者体内维生素 D 与 GPX4 和铁参数的关系。研究方法纳入慢性阻塞性肺病患者和对照组受试者。收集血清样本和肺组织。测定血清维生素 D 和铁的水平以及肺铁蛋白和 GPX4 的表达。此外,用 1,25(OH)2D3(100 nM)(维生素 D3 的活性形式)培养人肺上皮细胞(BEAS-2B)。然后检测维生素 D 受体(VDR)和核因子(红细胞衍生 2)样 2(Nrf-2)信号转导。结果显示慢性阻塞性肺病患者的血清 25- 羟维生素 D(25(OH)D)降低,血清和肺组织中的铁和铁蛋白水平升高。此外,肺部 GPX4 的表达也有所降低。相关分析表明,肺功能与铁参数成反比,而与 GPX4 成正比。结果表明,血清 25(OH)D 缺乏与血清铁参数升高和肺 GPX4 降低有关。此外,与对照组相比,慢性阻塞性肺病患者的 VDR 和 Nrf-2 阳性肺核减少。在慢性阻塞性肺病患者中,研究结果表明 VDR 和 Nrf-2 之间存在正相关关系。进一步分析发现,Nrf-2 阳性细胞核与铁参数呈负相关。体外实验发现,1,25(OH)2D3 治疗激活了 VDR 信号,并提高了 BEAS-2B 细胞中 Nrf-2 和 GPX4 的表达。结论维生素 D 缺乏与 COPD 患者 GPX4 减少和铁参数升高呈正相关。建议探讨补充维生素 D 在慢性阻塞性肺病进展中的作用。
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来源期刊
Canadian respiratory journal
Canadian respiratory journal 医学-呼吸系统
CiteScore
4.20
自引率
0.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Canadian Respiratory Journal is a peer-reviewed, Open Access journal that aims to provide a multidisciplinary forum for research in all areas of respiratory medicine. The journal publishes original research articles, review articles, and clinical studies related to asthma, allergy, COPD, non-invasive ventilation, therapeutic intervention, lung cancer, airway and lung infections, as well as any other respiratory diseases.
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