Global DNA methylation level in spermatozoa is not associated with ICSI fertilization outcome and embryo quality in donor oocyte programme.

IF 2.1 4区医学 Q2 OBSTETRICS & GYNECOLOGY Human Fertility Pub Date : 2024-12-01 Epub Date: 2024-11-06 DOI:10.1080/14647273.2024.2421191

Riddhi Kirit Pandya, Sujith Raj Salian, Vani R Lakshmi, Shubhashree Uppangala, Guruprasad Kalthur, Sanjay Gupta, Satish Kumar Adiga

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Abstract

Epigenetic mechanisms, including DNA methylation and histone modifications, govern chromatin arrangement in sperm, enhancing motility and safeguarding DNA integrity for accurate epigenetic inheritance. Abnormal methylation is linked to poor sperm quality and fertility issues, underscoring the need to study sperm DNA methylation and its impact on sperm function and embryo development in assisted reproductive technology. In this study, processed spermatozoa from 75 normozoospermic and 15 abnormal ejaculates were examined for sperm global DNA methylation levels using a colourimetric absorbance method. Although semen characteristics were poor in abnormal ejaculates, no significant correlation was found between sperm global DNA methylation levels and sperm characteristics in either normozoospermic or abnormal cohorts. However, mean global DNA methylation levels were significantly lowered in abnormal sperm samples compared to normozoospermic samples (p < 0.05). Furthermore, injecting spermatozoa from these patients (N = 50) into donor oocytes did not show a significant relationship between sperm global DNA methylation and embryo developmental competence. These findings highlight the limitation of sperm global DNA methylation as a biomarker for embryo development and quality.

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精子中的 DNA 甲基化水平与 ICSI 受精结果和供卵计划中的胚胎质量无关。

表观遗传机制（包括 DNA 甲基化和组蛋白修饰）控制着精子中染色质的排列，提高精子的运动能力并保护 DNA 的完整性，从而实现准确的表观遗传。异常甲基化与精子质量差和生育问题有关，这突出表明有必要研究精子 DNA 甲基化及其对精子功能和辅助生殖技术中胚胎发育的影响。在这项研究中，采用比色吸光法对来自 75 个正常无精子症患者和 15 个异常射精患者的精子进行了处理，以检测精子全球 DNA 甲基化水平。虽然异常射精的精液特征较差，但无论是正常无精症患者还是异常射精患者，精子全局 DNA 甲基化水平与精子特征之间都没有发现明显的相关性。然而，与正常无精子症样本相比，异常精子样本的平均全局 DNA 甲基化水平明显降低（p

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来源期刊

Human Fertility OBSTETRICS & GYNECOLOGY-REPRODUCTIVE BIOLOGY

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Human Fertility is a leading international, multidisciplinary journal dedicated to furthering research and promoting good practice in the areas of human fertility and infertility. Topics included span the range from molecular medicine to healthcare delivery, and contributions are welcomed from professionals and academics from the spectrum of disciplines concerned with human fertility. It is published on behalf of the British Fertility Society. The journal also provides a forum for the publication of peer-reviewed articles arising out of the activities of the Association of Biomedical Andrologists, the Association of Clinical Embryologists, the Association of Irish Clinical Embryologists, the British Andrology Society, the British Infertility Counselling Association, the Irish Fertility Society and the Royal College of Nursing Fertility Nurses Group. All submissions are welcome. Articles considered include original papers, reviews, policy statements, commentaries, debates, correspondence, and reports of sessions at meetings. The journal also publishes refereed abstracts from the meetings of the constituent organizations.