Enhanced Bacterial and Biofilm Adhesion Resistance of ALD Nano-TiO₂ Coatings Compared to AO Coatings on Titanium Abutments.

IF 6.6 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY International Journal of Nanomedicine Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI:10.2147/IJN.S482478

Yu Pan, Lili Cao, Libing Chen, Linjuan Gao, Xia Wei, Honglei Lin, Lei Jiang, Yinghui Wang, Hui Cheng

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Abstract

Purpose: The study was intended to compare the surface properties and the bacterial and biofilm adhesion resistance of two potential antibacterial nanometer titanium dioxide (nano-TiO₂) coatings on dental titanium (Ti) abutments prepared by atomic layer deposition (ALD) and the anodic oxidation (AO) techniques.

Methods: Nano-TiO₂ coatings were developed using ALD and AO techniques and applied to Ti surfaces. The surface properties and the bacterial and biofilm adhesion resistance of these coatings were evaluated against commonly used Ti and Zirconia (ZrO₂) surfaces. The chemical compositions, crystalline forms, surface topography, roughness and hydrophilicity were characterized. The antibacterial performance was assessed by the scanning electron microscope (SEM), the Colony-forming unit (CFU) assay and the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay using in vitro models of Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), and Porphyromonas gingivalis (P. gingivalis) in both single- and mixed-species bacterial compositions.

Results: ALD-prepared nano-TiO₂ coatings resulted in a dense, smooth, and less hydrophilic surface with an anatase phase, significantly reducing the adhesion of the three bacteria by over 50%, comparable to ZrO₂. In contrast, AO-prepared coatings led to a less hydrophilic surface, characterized by various nano-sized pores within the oxide film. This alteration, however, had no impact on the adhesion of the three bacteria. The adhesion patterns for mixed-species bacteria were generally consistent with single-species results.

Conclusion: ALD-prepared nano-TiO₂ coatings on Ti abutments demonstrated promising antibacterial properties comparable to ZrO₂ surfaces, suggesting potential in preventing peri-implantitis. However, the bacterial and biofilm adhesion resistance of AO-produced nano-TiO₂ coatings was limited.

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与钛基台上的 AO 涂层相比，ALD 纳米二氧化钛涂层具有更强的抗细菌和生物膜粘附性。

目的：本研究旨在比较通过原子层沉积（ALD）和阳极氧化（AO）技术制备的两种潜在抗菌纳米二氧化钛（Nano-TiO2）涂层在牙科钛（Ti）基台上的表面特性以及抗细菌和生物膜附着性：方法：采用原子层沉积（ALD）和阳极氧化（AO）技术制备了纳米二氧化钛涂层，并将其应用于钛基台表面。对照常用的钛和氧化锆（ZrO₂）表面，对这些涂层的表面特性以及抗细菌和生物膜附着性进行了评估。对涂层的化学成分、结晶形式、表面形貌、粗糙度和亲水性进行了表征。通过扫描电子显微镜（SEM）、菌落形成单位（CFU）检测法和 3-（4，5-二甲基噻唑基-2）-2，5-二苯基溴化四氮唑（MTT）检测法，使用金黄色葡萄球菌（S.金黄色葡萄球菌（S. aureus）、变异链球菌（S. mutans）和牙龈卟啉单胞菌（P. gingivalis）在单一菌种和混合菌种组成中的体外模型进行测定：结果：ALD 制备的纳米氧化钛涂层表面致密、光滑、亲水性较低且具有锐钛矿相，可显著降低这三种细菌 50%以上的粘附性，与氧化锆相当。相比之下，AO 制备的涂层表面亲水性较差，氧化膜中存在各种纳米级孔隙。不过，这种变化对三种细菌的附着力没有影响。混合菌种的粘附模式与单一菌种的结果基本一致：ALD制备的Ti基台上的纳米TiO₂涂层表现出了与ZrO₂表面相当的抗菌性能，这表明其在预防种植体周围炎方面具有潜力。然而，AO 生产的纳米₂ TiO₂涂层的抗细菌和生物膜附着能力有限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.