Rapid Characterization of the Constituents in Classic Chinese Medicine Formula Guyinjian Using Ultra-High-Performance Liquid Chromatography With Quadrupole Time-of-Flight Mass Spectrometry

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL Journal of separation science Pub Date : 2024-11-06 DOI:10.1002/jssc.70019

Gang Chen, Nan Ge, Le Yang, Hui Sun, Ye Sun, Guangli Yan, Ying Han, Xiang Li, Xijun Wang

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Abstract

Guyinjian (GYJ) is a classic traditional Chinese medicine formula for the treatment of polycystic ovary syndrome, ovulation disorders, immuno-sterility, oligomenorrhea with kidney deficiency, and so forth. Due to the unclear chemical components of GYJ, the elucidation of the pharmacological mechanism, and the comprehensive development and utilization of GYJ in clinical is being restricted. In this study, ultra-high-performance liquid chromatography with quadrupole time-of-flight-tandem mass spectrometry combined with the Progenesis QI platform and using reference standards, online and self-built databases matching, fragmentation rules analysis of mass spectrometry peaks, a total of 235 compounds were preliminarily characterized, including 69 flavonoids, 65 terpenoids, 34 phenylpropanoids, 22 saccharides, sugar esters, and glycosides, 15 organic acids, and 30 others. These compounds may reflect the chemical properties of GYJ. The method established in this study can systematically, rapidly, and accurately resolve the chemical components in GYJ, which lays the foundation for further establishment of the pharmacodynamic substance basis and quality control.

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利用超高效液相色谱-四极杆飞行时间质谱法快速表征经典中药古银翘散中的成分

养阴鉴（GYJ）是治疗多囊卵巢综合征、排卵障碍、免疫性不孕、肾虚性闭经等疾病的经典中药方剂。由于GYJ的化学成分不明确，其药理机制的阐明和临床综合开发利用受到限制。本研究采用超高效液相色谱-四极杆飞行时间-串联质谱法，结合 Progenesis QI 平台，通过参考标准、在线数据库和自建数据库比对、质谱峰的碎片规则分析，初步定性了 235 种化合物，其中包括 69 种黄酮类化合物，65 种萜类化合物，34 种苯丙类化合物，22 种糖、糖酯和糖苷类化合物，15 种有机酸类化合物，以及 30 种其他化合物。这些化合物可能反映了 GYJ 的化学特性。本研究建立的方法能系统、快速、准确地解析 GYJ 中的化学成分，为进一步建立药效物质基础和质量控制奠定了基础。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.