Rapid Characterization and Identification of the Chemical Constituents in San-Huang-Xie-Xin-Tang, a Traditional Chinese Medicine Formula, by High Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-11-05 DOI:10.1093/chromsci/bmae056

Lei Liu, Pei Hu, Mengmo Chen, Jiasi Huang, Qing Zhang, Yun Ling

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Abstract

San-Huang-Xie-Xin-Tang (SHXXT), a traditional Chinese medicine formula composed of Coptidis rhizome (CR), Scutellariae radix (SR) and Rhei rhizome (RR), is widely used to treat hypertension, gastritis and peptic ulcer clinically. However, the chemical constituent of SHXXT is still unclear due to its complexity, which hindered the discovery of effective compounds and quality control. Thus, an in-depth understanding of its chemical constituents is very essential. To address this problem, comprehensive analysis of chemical constituents of SHXXT was performed by high performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS). As a result, 45 compounds were identified, including 8 phenolic acids, 15 flavonoids, 13 alkaloids, 8 anthraquinones and 1 lignan. Among them, 17 compounds were from CR, 17 compounds were from SR and 11 compounds were from RR. This established method can systematically and rapidly analyze the chemical constituents in SHXXT, which provides chemical foundation for further research on effective substances and action mechanism of SHXXT.

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高效液相色谱-电喷雾离子化和四极杆飞行时间质谱法快速表征和鉴定中药配方三黄解毒汤中的化学成分

由黄连、黄芩和大黄组成的中药三黄散（SHXXT）在临床上被广泛用于治疗高血压、胃炎和消化性溃疡。然而，由于其化学成分的复杂性，SHXXT 的化学成分仍不清楚，这阻碍了有效化合物的发现和质量控制。因此，深入了解其化学成分非常必要。针对这一问题，我们采用高效液相色谱-电喷雾离子化和四极杆飞行时间质谱（HPLC-ESI-QTOF-MS/MS）对 SHXXT 的化学成分进行了全面分析。结果鉴定出 45 种化合物，包括 8 种酚酸类化合物、15 种黄酮类化合物、13 种生物碱类化合物、8 种蒽醌类化合物和 1 种木脂素类化合物。其中，17 个化合物来自 CR，17 个化合物来自 SR，11 个化合物来自 RR。该方法能系统、快速地分析石杉碱甲中的化学成分，为进一步研究石杉碱甲的有效物质和作用机理提供了化学基础。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.