Host trees partially explain the complex bacterial communities of two threatened saproxylic beetles.

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-11-06 DOI:10.1111/imb.12973
Michał Kolasa, Rama Sarvani Krovi, Radosław Plewa, Tomasz Jaworski, Marcin Kadej, Adrian Smolis, Jerzy M Gutowski, Krzysztof Sućko, Rafał Ruta, Tomasz Olbrycht, Sergey Saluk, Maria Oczkowicz, Łukasz Kajtoch
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Abstract

Microorganisms are integral to ecosystem functioning and host adaptation, yet the understanding of microbiomes in diverse beetle taxa remains limited. We conducted a comprehensive study to investigate the microbial composition of two red flat bark beetle species, Cucujus haematodes and C. cinnaberinus, and assessed the influence of host taxonomic relatedness and host tree species on their microbiomes. We sampled 67 larvae of two Cucujus taxa taken from 11 host tree species. 16S rRNA V4 fragment sequencing revealed distinct microbial communities associated with each Cucujus species, with host tree species significantly influencing microbiome composition. Alpha and beta diversity metrics indicated significant differences between microbial communities in both beetle and host tree species. Principal component analysis indicated distinct clustering based on host tree species but not for beetle species. This overlap could be attributed to the similar ecology of both Cucujus species. The detection of various bacteria, among which some have already been reported in saproxylophagous beetles, suggests that the red flat bark beetles ingest the bacteria via foraging on other wood-dwelling invertebrates. Our findings show the complex interplay between host taxonomy, microhabitat and microbial composition in Cucujus, providing insights into their ecological roles and conservation implications. This research helps to fill the gap in understanding the microbial dynamics of saproxylic beetles, sheds light on factors shaping their microbiomes and highlights the importance of considering both host species and environmental conditions when studying insect-microbe interactions in forest ecosystems.

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寄主树部分解释了两种濒危甲虫复杂的细菌群落。
微生物是生态系统功能和宿主适应性不可或缺的组成部分,但人们对不同甲虫类群微生物组的了解仍然有限。我们进行了一项综合研究,调查了两种红扁柏甲虫(Cucujus haematodes和C. cinnaberinus)的微生物组成,并评估了寄主分类相关性和寄主树种对其微生物组的影响。我们从 11 个寄主树种中采集了两个 Cucujus 类群的 67 个幼虫样本。16S rRNA V4 片段测序结果显示,每个葫芦科物种都有不同的微生物群落,寄主树种对微生物组的组成有显著影响。阿尔法和贝塔多样性指标表明,甲虫和寄主树种的微生物群落之间存在显著差异。主成分分析表明,寄主树种有明显的聚类,而甲虫树种则没有。这种重叠可能是由于两种 Cucujus 树种的生态环境相似。红扁柏甲虫体内发现了多种细菌,其中一些细菌已在食脂甲虫体内被报道过,这表明红扁柏甲虫是通过觅食其他木栖无脊椎动物而摄入这些细菌的。我们的研究结果表明,宿主分类、微生境和Cucujus中的微生物组成之间存在复杂的相互作用,这为我们了解它们的生态作用和保护意义提供了启示。这项研究有助于填补人们对脂腺甲虫微生物动态了解的空白,揭示了影响其微生物组的因素,并强调了在研究森林生态系统中昆虫与微生物相互作用时同时考虑寄主物种和环境条件的重要性。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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