Manuka Honey Inhibits Biofilm Formation and Reduces the Expression of the Associated Genes in Pectobacterium brasiliense.

IF 2.3 Q2 BIOLOGY Scientifica Pub Date : 2024-10-28 eCollection Date: 2024-01-01 DOI:10.1155/2024/8837149
Tri Joko, Sheila Ava, Isna Nurifa Sasmita Putri, Siti Subandiyah, Muhammad Saifur Rohman, Naoto Ogawa
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引用次数: 0

Abstract

Biofilms are major virulence factors formed by pathogenic bacteria to invade their host and maintain their colony. While biofilms usually develop on diverse solid surfaces, floating biofilms, also called pellicles, are formed at the air-liquid interface. To address the problem of biofilm formation by bacterial pathogens, honey has been extensively studied. However, information on the effect of honey on biofilm formation by plant pathogens is scarce. This study aimed to determine the effects of manuka honey on biofilm and pellicle formation by Pectobacterium brasiliense and analyze the expression of genes encoding proteins needed to form biofilm by using semiquantitative PCR and RT-qPCR. Treatment with 5% (w/v) of manuka honey significantly decreased biofilm and pellicle formation by P. brasiliense. RT-qPCR results showed that the expression of bcsA, fis, hrpL, and expI decreased 7.07-fold, 5.71-fold, 13.11-fold, and 6.26-fold, respectively, after exposure to 5% (w/v) manuka honey. Our findings reveal that manuka honey may effectively inhibit biofilm and pellicle formation.

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麦卢卡蜂蜜可抑制生物膜的形成并减少巴西果胶杆菌相关基因的表达。
生物膜是病原菌入侵宿主和维持菌落的主要毒力因子。生物膜通常在不同的固体表面形成,而漂浮的生物膜(也称为胶粒)则在空气-液体界面形成。为了解决细菌病原体形成生物膜的问题,人们对蜂蜜进行了广泛的研究。然而,有关蜂蜜对植物病原体形成生物膜的影响的资料却很少。本研究旨在确定麦卢卡蜂蜜对巴西果胶杆菌形成生物膜和胶粒的影响,并采用半定量 PCR 和 RT-qPCR 分析编码形成生物膜所需蛋白质的基因的表达。用 5%(w/v)的麦卢卡蜂蜜处理后,能明显减少巴西栉水母杆菌生物膜和胶粒的形成。RT-qPCR结果显示,暴露于5%(w/v)的麦卢卡蜂蜜后,bcsA、fis、hrpL和expI的表达量分别下降了7.07倍、5.71倍、13.11倍和6.26倍。我们的研究结果表明,麦卢卡蜂蜜可有效抑制生物膜和胶粒的形成。
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来源期刊
Scientifica
Scientifica BIOLOGY-
CiteScore
6.70
自引率
0.00%
发文量
43
审稿时长
21 weeks
期刊介绍: Scientifica is a peer-reviewed, Open Access journal that publishes research articles, review articles, and clinical studies covering a wide range of subjects in the life sciences, environmental sciences, health sciences, and medicine. The journal is divided into the 65 subject areas.
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