Decreased Serum Insulin Receptor Messenger RNA Level in H. pylori IgG Seropositive Type 2 Diabetic Patients.

IF 3.4 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Biomarker Insights Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI:10.1177/11772719241296619
Emmanuel Ayitey Tagoe, Jael Acquah Appiah, Pius Agyenim Boateng, Osbourne Quaye, Samuel Bosomprah
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Abstract

Background: Helicobacter pylori (H. pylori) is a known gastro-intestinal pathogen but implicated in extra-gastric diseases. The relationship between H. pylori infection and type 2 diabetes (T2DM) remains insufficiently elucidated, particularly in terms of molecular mediators such as microRNAs (miRNAs) and messenger RNAs (mRNAs).

Objective: We aimed to characterize expression pattern of insulin signalling mRNAs and targeted miRNAs in T2DM patients exposed to H. pylori infection.

Methods: We conducted a cross-sectional study among patients diagnosed with type 2 diabetes mellitus and were aged 18 to 60 years. Overnight fasting blood samples were collected and processed for plasma and serum. The plasma samples were used for glucose estimation and the serum used for H. pylori IgG screening. Total RNA was extracted from the serum with commercial kit, and mRNAs and miRNAs quantified by RT-qPCR with specific primers and under predetermined amplification conditions. Clinical data were obtained from medical records of patients.

Results: Among 351 patients enrolled, 267 (76.1%) were females, 224 (63.8%) were married, and 79 (22.5%) had tertiary education. Expression level of insulin receptor mRNA was significantly lower in H. pylori positive T2DM patients compared to H. pylori negative (P < .05). There was no evidence of a difference in insulin receptor substrate 1 mRNA level (P > .05). Although not statistically significant, the expression levels of miRNA-222 and miRNA-155 in the patients exposed to H. pylori were higher than that of the unexposed group (P > .05).

Conclusions: We found a significantly reduced serum insulin receptor messenger RNA level and higher levels of miRNA-222 and miRNA-155 in H. pylori exposed T2DM patients. The findings suggest a possible role of the infection in insulin signalling alteration in the patients.

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幽门螺杆菌 IgG 血清阳性 2 型糖尿病患者血清胰岛素受体信使 RNA 水平降低
背景:幽门螺杆菌(H. pylori)是一种已知的胃肠道病原体,但也与胃肠道以外的疾病有关。幽门螺杆菌感染与 2 型糖尿病(T2DM)之间的关系仍未得到充分阐明,尤其是在分子介质方面,如微小核糖核酸(miRNA)和信使核糖核酸(mRNA):我们的目的是描述幽门螺杆菌感染的 T2DM 患者中胰岛素信号 mRNA 和靶向 miRNA 的表达模式:我们对确诊为 2 型糖尿病的 18 至 60 岁患者进行了一项横断面研究。我们采集了隔夜空腹血样,并对血浆和血清进行了处理。血浆样本用于估算血糖,血清样本用于筛查幽门螺杆菌 IgG。使用商业试剂盒从血清中提取总 RNA,并在预定的扩增条件下使用特定引物通过 RT-qPCR 对 mRNA 和 miRNA 进行定量。临床数据来自患者的医疗记录:在 351 例患者中,267 例(76.1%)为女性,224 例(63.8%)已婚,79 例(22.5%)受过高等教育。幽门螺杆菌阳性 T2DM 患者的胰岛素受体 mRNA 表达水平明显低于幽门螺杆菌阴性患者(P P > .05)。暴露于幽门螺杆菌的患者中,miRNA-222和miRNA-155的表达水平高于未暴露组,但无统计学意义(P > .05):结论:我们发现暴露于幽门螺杆菌的 T2DM 患者血清胰岛素受体信使 RNA 水平明显降低,miRNA-222 和 miRNA-155 水平较高。这些研究结果表明,幽门螺杆菌感染可能会改变患者体内的胰岛素信号。
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来源期刊
Biomarker Insights
Biomarker Insights MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.00
自引率
0.00%
发文量
26
审稿时长
8 weeks
期刊介绍: An open access, peer reviewed electronic journal that covers all aspects of biomarker research and clinical applications.
期刊最新文献
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