Synbiotic Effects of Lacticaseibacillus paracasei K56 and Prebiotics on the Intestinal Microecology of Children with Obesity.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Probiotics and Antimicrobial Proteins Pub Date : 2024-11-06 DOI:10.1007/s12602-024-10395-0

Pengwei Zhang, Xianhui Dong, Yijun Zeng, Junkui Chen, Sijia Yang, Peipei Yu, Chunhong Ye, Wei-Lian Hung, Qiuyue Jiang, Wen Zhao, Zhaozhong Zeng, Jinjun Li, Li Li

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Abstract

Lacticaseibacillus paracasei K56 (L. paracasei K56) is a probiotic with weight-loss effects. However, symbiosis research on the combined effects of Lacticaseibacillus paracasei K56 and prebiotics is lacking. Therefore, the aim of this study was to investigate the effects of L. paracasei K56, xylooligosaccharide (XOS), galactooligosaccharide (GOS), polyglucose (PG), and their synbiotic combinations (XOS + K56, GOS + K56, and PG + K56) on metabolism and gut composition in children with obesity, using an in vitro fermentation model. Fecal samples were collected from 14 children with obesity for in vitro fermentation, and the effects of the various treatments in gas production and short chain fatty acid synthesis (SCFAs) were assessed. Treatment with probiotics, prebiotics, and synbiotics regulated gut microbiota and metabolites in children with obesity. GOS and XOS had higher degradation rates than PG + K56 synbiotics in the gut microbiota of children with obesity. Moreover, treatment with XOS, GOS, and their synbiotic combinations, (XOS + K56) and (GOS + K56), significantly reduced the production of gas, propionic acid, and butyric acid compared with PG + K56 treatment. Treatments with GOS + K56 and XOS + K56 altered the composition of the gut microbiota, improved the abundance of Bifidobacteria and Lactobacilli, and reduced the abundance of Escherichia/Shigella. Overall, this study provides a theoretical foundation for the use of K56-based synbiotics.

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副酸乳杆菌 K56 和益生元对肥胖儿童肠道微生态的协同作用

副酸乳杆菌 K56（L. paracasei K56）是一种具有减肥功效的益生菌。然而，有关副酸性乳杆菌 K56 和益生元共同作用的共生研究还很缺乏。因此，本研究旨在利用体外发酵模型，研究副酸乳杆菌 K56、木寡糖（XOS）、半乳寡糖（GOS）、多聚葡萄糖（PG）以及它们的益生元组合（XOS + K56、GOS + K56 和 PG + K56）对肥胖症儿童新陈代谢和肠道组成的影响。收集了 14 名肥胖症儿童的粪便样本进行体外发酵，并评估了各种处理对气体产生和短链脂肪酸合成（SCFAs）的影响。益生菌、益生元和合成益生菌能调节肥胖症儿童的肠道微生物群和代谢物。在肥胖症儿童的肠道微生物群中，GOS 和 XOS 的降解率高于 PG + K56 合生素。此外，与 PG + K56 处理相比，XOS、GOS 及其合生素组合（XOS + K56）和（GOS + K56）处理可显著减少气体、丙酸和丁酸的产生。GOS + K56 和 XOS + K56 的处理改变了肠道微生物群的组成，提高了双歧杆菌和乳酸杆菌的数量，降低了埃希氏菌/志贺氏菌的数量。总之，这项研究为使用基于 K56 的益生菌提供了理论基础。

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.