Micro-CT study of human laryngeal structures using phosphotungstic agent staining.

IF 1.4 4区医学 Q2 Medicine Surgical and Radiologic Anatomy Pub Date : 2024-11-06 DOI:10.1007/s00276-024-03489-5

Kyu-Ho Yi, Hyung-Jin Lee, Ji-Hyun Lee

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Abstract

Purpose: Traditional dissection methods are primarily limited by challenges in identifying minute structures, which can lead to irreversible tissue damage. Anatomical observation of the larynx is particularly challenging in educational and clinical settings owing to its microscopic structures and complex three-dimensional (3D) nature, making it difficult to dissect. Therefore, this study aimed to demonstrate that micro-computed tomography (micro-CT) imaging of the larynx can serve as an effective alternative for educational and clinical purposes, overcoming these limitations.

Methods: Three laryngeal specimens were obtained from cadavers, stained with a phosphotungstic acid-based contrast agent, and imaged using enhanced micro-CT. The resulting images were reconstructed in three dimensions, allowing for a detailed 3D observation of the specimens.

Results: Phosphotungstic contrast-enhanced micro-CT provided comprehensive anatomical information on laryngeal structures, including muscles, nerves, arteries, and vocal folds.

Conclusion: This study demonstrates the high effectiveness of micro-CT in producing detailed structural images of the larynx, enabling 3D observation of even the smallest anatomical structures. These images can be applied in both educational and clinical settings to analyze the human larynx, effectively overcoming the limitations of traditional dissection methods. This approach facilitates the analysis of laryngeal structures that are otherwise difficult to observe with the naked eye.

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利用磷钨系染色剂对人类喉部结构进行显微 CT 研究。

目的：传统的解剖方法主要受限于识别微小结构的挑战，这可能导致不可逆的组织损伤。在教育和临床环境中，喉部的解剖观察尤其具有挑战性，因为喉部结构微小且具有复杂的三维（3D）特性，因此很难解剖。因此，本研究旨在证明喉部显微计算机断层扫描（micro-CT）成像可作为教育和临床目的的有效替代方法，克服这些局限性：方法：从尸体上获取三个喉部标本，用磷钨酸造影剂染色，并使用增强型微型计算机断层扫描成像。结果：磷钨酸造影剂增强显微计算机断层扫描对三维图像进行了重建，从而对标本进行了详细的三维观察：结果：磷钨酸造影剂增强显微 CT 提供了喉部结构的全面解剖信息，包括肌肉、神经、动脉和声带：这项研究表明，显微 CT 在制作喉部详细结构图像方面具有很高的效率，即使是最微小的解剖结构也能进行三维观察。这些图像可用于教育和临床环境中分析人类喉部，有效克服了传统解剖方法的局限性。这种方法有助于分析肉眼难以观察到的喉部结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Surgical and Radiologic Anatomy Medicine-Pathology and Forensic Medicine

CiteScore

2.40

自引率

14.30%

发文量

期刊介绍： Anatomy is a morphological science which cannot fail to interest the clinician. The practical application of anatomical research to clinical problems necessitates special adaptation and selectivity in choosing from numerous international works. Although there is a tendency to believe that meaningful advances in anatomy are unlikely, constant revision is necessary. Surgical and Radiologic Anatomy, the first international journal of Clinical anatomy has been created in this spirit. Its goal is to serve clinicians, regardless of speciality-physicians, surgeons, radiologists or other specialists-as an indispensable aid with which they can improve their knowledge of anatomy. Each issue includes: Original papers, review articles, articles on the anatomical bases of medical, surgical and radiological techniques, articles of normal radiologic anatomy, brief reviews of anatomical publications of clinical interest. Particular attention is given to high quality illustrations, which are indispensable for a better understanding of anatomical problems. Surgical and Radiologic Anatomy is a journal written by anatomists for clinicians with a special interest in anatomy.