Preliminary Characterization of the Vasorelaxant Effect of Thymus atlanticus (Ball) Roussine Using Optical Methods.

Hamza Elbouny, Nabil Bouchebchoub, Brahim Ouahzizi, Rania Hajjami, Younes Filali-Zegzouti, Mohamed Yassine Amarouch, Chakib Alem
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Abstract

Background: Thymus atlanticus (Ball) Roussine is a Moroccan endemic thyme species that is traditionally used as an aromatic and medicinal plant. Several studies have demonstrated its pharmacological significance and therapeutic value.

Objective: The current study aimed to assess the vasorelaxant effect of the aqueous extract of this species.

Methods: The contractility of isolated rat aortas was investigated using the multi-well organ bath technique. This method was adapted and validated in our experimental conditions using epinephrine and hydralazine as vasoconstrictive and vasodilator agents, respectively. The application of 10 μM epinephrine induced a clear vasoconstriction of the aorta rings (Lumen reduction = 31.8±0.4%). However, hydralazine induced a dose-dependent relaxation with an EC50 value of 6.1±1.2 mM. For the aqueous extract of T. atlanticus, the aortic rings were precontracted with epinephrine, and then increasing concentrations (0.125-1 mg/mL) of this extract were added cumulatively.

Results: The results have indicated T. atlanticus extract to have a significant vasodilatory effect in a dose-dependent manner (EC50 = 0.52±0.03 mg/mL).

Conclusion: The findings provide preliminary evidence of the vasorelaxant effect of the aqueous extract of T. atlanticus using a low-cost optical approach. However, the cellular and molecular mechanisms underlying this effect have yet to be revealed.

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用光学方法初步鉴定大西洋胸腺(波尔)鲁辛的血管舒张效果
背景:Thymus atlanticus (Ball) Roussine 是摩洛哥特有的百里香品种,传统上被用作芳香植物和药用植物。多项研究证明了其药理意义和治疗价值:本研究旨在评估该物种水提取物的血管舒张作用:方法:采用多孔器官浴技术研究了离体大鼠主动脉的收缩能力。在我们的实验条件下,使用肾上腺素和肼屈嗪分别作为血管收缩剂和血管扩张剂,对该方法进行了调整和验证。应用 10 μM 肾上腺素可引起主动脉环明显的血管收缩(管腔缩小 = 31.8±0.4%)。然而,肼屈嗪可诱导剂量依赖性松弛,其 EC50 值为 6.1±1.2 mM。对于大西洋金枪鱼的水提取物,先用肾上腺素预收缩主动脉环,然后逐渐加入浓度越来越高(0.125-1 mg/mL)的该提取物:结果:结果表明,大西洋金枪鱼提取物具有显著的血管扩张作用,且呈剂量依赖性(EC50 = 0.52±0.03 mg/mL):结论:研究结果提供了使用低成本光学方法检测大西洋金枪鱼水提取物的血管舒张效果的初步证据。然而,这种作用的细胞和分子机制仍有待揭示。
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