A widespread phage-encoded kinase enables evasion of multiple host antiphage defence systems

IF 20.5 1区 生物学 Q1 MICROBIOLOGY Nature Microbiology Pub Date : 2024-11-06 DOI:10.1038/s41564-024-01851-2
Susu Jiang, Chao Chen, Wanqiu Huang, Yue He, Xuan Du, Yi Wang, Hongda Ou, Zixin Deng, Congrui Xu, Lixu Jiang, Lianrong Wang, Shi Chen
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Abstract

DNA degradation (Dnd) is a widespread bacterial antiphage defence system that relies on DNA phosphorothioate (PT) modification for self/non-self discrimination and subsequent degradation of unmodified DNA. Phages employ counterstrategies to evade host immunity, but anti-Dnd immunity has not been characterized. Here we report an immune evasion protein encoded by the Salmonella phage JSS1 that contributes to subverting Dnd and other defence systems. Using quantitative proteomic and phosphoproteomic analyses, we show that the protein JSS1_004 employs N-terminal Ser/Thr/Tyr protein kinase activity to catalyse the multisite phosphorylation of host DndFGH. Notably, JSS1_004 also phosphorylates other bacterial immune systems to varying degrees, including CRISPR‒Cas, QatABCD, SIR2+HerA and DUF4297+HerA. Given that JSS1_004 and its homologues are widespread in phylogenetically diverse phages, we suggest that this strategy constitutes a family of immune evasion proteins that increases the chances of phage proliferation even when a host deploys multiple defence systems.

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一种广泛存在的噬菌体编码激酶能够躲避多种宿主抗噬菌体防御系统的攻击
DNA 降解(Dnd)是一种广泛存在的细菌抗噬菌体防御系统,它依靠硫代磷酸 DNA(PT)修饰来区分自体/非自体,并随后降解未修饰的 DNA。噬菌体采用反策略来逃避宿主免疫,但抗 Dnd 免疫尚未定性。在这里,我们报告了沙门氏菌噬菌体 JSS1 编码的一种免疫逃避蛋白,它有助于颠覆 Dnd 和其他防御系统。通过定量蛋白质组学和磷酸化蛋白质组学分析,我们发现 JSS1_004 蛋白利用 N 端 Ser/Thr/Tyr 蛋白激酶活性催化宿主 DndFGH 的多位点磷酸化。值得注意的是,JSS1_004 还能在不同程度上磷酸化其他细菌免疫系统,包括 CRISPR-Cas、QatABCD、SIR2+HerA 和 DUF4297+HerA。鉴于 JSS1_004 及其同源物广泛存在于系统发育多样的噬菌体中,我们认为这种策略构成了一个免疫逃避蛋白家族,即使宿主部署了多种防御系统,它也能增加噬菌体增殖的机会。
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来源期刊
Nature Microbiology
Nature Microbiology Immunology and Microbiology-Microbiology
CiteScore
44.40
自引率
1.10%
发文量
226
期刊介绍: Nature Microbiology aims to cover a comprehensive range of topics related to microorganisms. This includes: Evolution: The journal is interested in exploring the evolutionary aspects of microorganisms. This may include research on their genetic diversity, adaptation, and speciation over time. Physiology and cell biology: Nature Microbiology seeks to understand the functions and characteristics of microorganisms at the cellular and physiological levels. This may involve studying their metabolism, growth patterns, and cellular processes. Interactions: The journal focuses on the interactions microorganisms have with each other, as well as their interactions with hosts or the environment. This encompasses investigations into microbial communities, symbiotic relationships, and microbial responses to different environments. Societal significance: Nature Microbiology recognizes the societal impact of microorganisms and welcomes studies that explore their practical applications. This may include research on microbial diseases, biotechnology, or environmental remediation. In summary, Nature Microbiology is interested in research related to the evolution, physiology and cell biology of microorganisms, their interactions, and their societal relevance.
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