Synergistic effect of curcumin and Piperine loaded Niosomal nanoparticles on acute pulmonary toxicity induced by Paraquat in mice.

IF 2.2 4区医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-11-05 eCollection Date: 2024-12-01 DOI:10.1093/toxres/tfae181

Aram Khederzadeh, Pedram Ebrahimnejad, Mohammad Seyedabadi, Amirhossein Babaei, Fereshteh Talebpour Amiri, Nasim Aslani, Solmaz Mojarad-Jabali, Hamidreza Mohammadi

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Abstract

Objective: Paraquat (PQ), a widely used non-selective herbicide, induces severe lung toxicity by promoting cell death and tissue necrosis through the generation of reactive oxygen species (ROS) and free radicals. This study aimed to develop and evaluate novel niosomal nanoparticles (NPs) encapsulating curcumin and piperine to mitigate PQ-induced acute pulmonary toxicity in Balb/c mice.

Methods: The NPs were prepared using non-ionic surfactants and cholesterol via the thin film hydration method.

Results: Characterization revealed high encapsulation efficiency (>85%), proper particle sizes (264-286 nm), narrow polydispersity index (PDI) (0.19 ± 0.04 to 0.23 ± 0.02), and good stability over 90 days. Thermal analysis confirmed successful encapsulation of curcumin and piperine within the niosomal NPs. In vivo studies showed that PQ exposure significantly elevated ROS, lipid peroxidation (LPO), and protein carbonylation (PC) levels, while reducing glutathione (GSH) levels and impairing mitochondrial function (P < 0.001). However, co-treatment with curcumin- and piperine-loaded niosomal NPs effectively reversed these effects (P < 0.001), improving mitochondrial function.

Conclusion: The combined formulation of curcumin and piperine in niosomal NPs offers a promising therapeutic strategy for treating PQ-induced pulmonary toxicity, likely due to enhanced bioavailability and potent antioxidant activity.

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姜黄素和胡椒碱负载的 Niosomal 纳米粒子对百草枯诱导的小鼠急性肺毒性的协同作用

目的：百草枯（PQ）是一种广泛使用的非选择性除草剂：百草枯（PQ）是一种广泛使用的非选择性除草剂，通过产生活性氧（ROS）和自由基促进细胞死亡和组织坏死，从而诱发严重的肺毒性。本研究旨在开发和评估包裹姜黄素和胡椒碱的新型纳米颗粒（NPs），以减轻 PQ 诱导的 Balb/c 小鼠急性肺毒性：方法：使用非离子表面活性剂和胆固醇，通过薄膜水合法制备 NPs：表征结果表明：NPs 的封装效率高（>85%），粒径大小合适（264-286 nm），多分散指数（PDI）较窄（0.19 ± 0.04 至 0.23 ± 0.02），90 天内稳定性良好。热分析证实了姜黄素和胡椒碱在纳米囊状 NPs 中的成功封装。体内研究表明，PQ 暴露会显著提高 ROS、脂质过氧化（LPO）和蛋白质羰基化（PC）水平，同时降低谷胱甘肽（GSH）水平并损害线粒体功能（P 结论）：姜黄素和胡椒碱在纳米级 NPs 中的组合制剂为治疗 PQ 诱导的肺毒性提供了一种很有前景的治疗策略，这可能是由于其生物利用度提高和具有强大的抗氧化活性。

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