Effects of different quorum sensing signal molecules on alleviation of ammonia inhibition during biomethanation

IF 7.7 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Environmental Research Pub Date : 2024-11-05 DOI:10.1016/j.envres.2024.120295
Siyi Chen , Ziang Kong , Liwei Qiu , Han Wang , Qun Yan
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Abstract

Anaerobic digestion (AD) is a promising technology for achieving both organic wastes treatment and energy recovery. However, challenges such as ammonia inhibition still remain. Quorum sensing (QS) system is relevant with the regulation of microbial community behaviors by releasing and sensing signal molecules, which could improve methane production during AD process. Therefore, the current study explored the effects of different quorum sensing signal molecules on alleviation of ammonia inhibition. The results showed that both secretion of N-butyryl-DL-homoserine lactone (C4-HSL) and N-(β-ketocaproyl)-DL-homoserine lactone (3OC6-HSL) could be inhibited by high ammonia stress while stimulation of N-hexanoyl-L-homoserine lactone (C6-HSL) and N-octanoyl-DL-homoserine lactone (C8-HSL) secretion might be triggered by ammonia toxicity. Moreover, the alleviation of ammonia inhibition could be achieved by both introducing 3OC6-HSL (0.5 μM) and combination of 3OC6-HSL (0.1 μM) and biochar (4 g/L). Exogenous 3OC6-HSL could regulate microbial social behaviors and enhance the secretion of extracellular polymeric substances (EPS) to promote anaerobic digestion. In addition, the mitigation of ammonia inhibition through exogenous 3OC6-HSL and biochar were confirmed by microbial community changes (Methanobacterium, Propionicicella and Petrimonas). Critical enzymes involved in both acidification and methanogenic steps were enhanced after adding the combination of 3OC6-HSL and biochar. The combination of low levels of 3OC6-HSL and biochar could promote both direct interspecies electron transfer (DIET) process and communication between different anaerobic microorganisms to mitigate ammonia inhibition. The current study will provide primary insights for conquering ammonia inhibition during biomethanation.

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不同的定量感应信号分子对减轻生物甲烷化过程中氨抑制作用的影响。
厌氧消化(AD)是一项很有前途的技术,既能处理有机废物,又能回收能源。然而,氨抑制等挑战依然存在。法定量感应(QS)系统通过释放和感应信号分子来调节微生物群落行为,从而提高厌氧消化过程中的甲烷产量。因此,本研究探讨了不同法定量传感信号分子对缓解氨抑制的影响。结果表明,高氨胁迫可抑制 N-丁酰基-DL-高丝氨酸内酯(C4-HSL)和 N-(β-酮酰基)-DL-高丝氨酸内酯(3OC6-HSL)的分泌,而氨毒性可能会刺激 N-己酰基-L-高丝氨酸内酯(C6-HSL)和 N-辛酰基-DL-高丝氨酸内酯(C8-HSL)的分泌。此外,通过引入 3OC6-HSL (0.5 μM)和 3OC6-HSL (0.1 μM)与生物炭(4 克/升)的组合,可以缓解氨抑制作用。外源 3OC6-HSL 可调节微生物的社会行为,促进胞外聚合物物质(EPS)的分泌,从而促进厌氧消化。此外,微生物群落(甲烷杆菌、丙酸杆菌和 Petrimonas)的变化也证实了外源 3OC6-HSL 和生物炭对氨抑制的缓解作用。添加 3OC6-HSL 和生物炭后,参与酸化和甲烷生成步骤的关键酶得到了增强。低浓度的 3OC6-HSL 与生物炭的结合可促进种间直接电子传递(DIET)过程和不同厌氧微生物之间的交流,从而减轻氨抑制作用。本研究将为克服生物甲烷化过程中的氨抑制问题提供初步见解。
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来源期刊
Environmental Research
Environmental Research 环境科学-公共卫生、环境卫生与职业卫生
CiteScore
12.60
自引率
8.40%
发文量
2480
审稿时长
4.7 months
期刊介绍: The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.
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