Siyi Chen , Ziang Kong , Liwei Qiu , Han Wang , Qun Yan
{"title":"Effects of different quorum sensing signal molecules on alleviation of ammonia inhibition during biomethanation","authors":"Siyi Chen , Ziang Kong , Liwei Qiu , Han Wang , Qun Yan","doi":"10.1016/j.envres.2024.120295","DOIUrl":null,"url":null,"abstract":"<div><div>Anaerobic digestion (AD) is a promising technology for achieving both organic wastes treatment and energy recovery. However, challenges such as ammonia inhibition still remain. Quorum sensing (QS) system is relevant with the regulation of microbial community behaviors by releasing and sensing signal molecules, which could improve methane production during AD process. Therefore, the current study explored the effects of different quorum sensing signal molecules on alleviation of ammonia inhibition. The results showed that both secretion of <em>N</em>-butyryl-<sub>DL</sub>-homoserine lactone (C4-HSL) and <em>N</em>-(β-ketocaproyl)-<sub>DL</sub>-homoserine lactone (3OC6-HSL) could be inhibited by high ammonia stress while stimulation of <em>N</em>-hexanoyl-<sub>L</sub>-homoserine lactone (C6-HSL) and <em>N</em>-octanoyl-<sub>DL</sub>-homoserine lactone (C8-HSL) secretion might be triggered by ammonia toxicity. Moreover, the alleviation of ammonia inhibition could be achieved by both introducing 3OC6-HSL (0.5 μM) and combination of 3OC6-HSL (0.1 μM) and biochar (4 g/L). Exogenous 3OC6-HSL could regulate microbial social behaviors and enhance the secretion of extracellular polymeric substances (EPS) to promote anaerobic digestion. In addition, the mitigation of ammonia inhibition through exogenous 3OC6-HSL and biochar were confirmed by microbial community changes (<em>Methanobacterium</em>, <em>Propionicicella</em> and <em>Petrimonas</em>). Critical enzymes involved in both acidification and methanogenic steps were enhanced after adding the combination of 3OC6-HSL and biochar. The combination of low levels of 3OC6-HSL and biochar could promote both direct interspecies electron transfer (DIET) process and communication between different anaerobic microorganisms to mitigate ammonia inhibition. The current study will provide primary insights for conquering ammonia inhibition during biomethanation.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"264 ","pages":"Article 120295"},"PeriodicalIF":7.7000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Research","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0013935124022023","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Anaerobic digestion (AD) is a promising technology for achieving both organic wastes treatment and energy recovery. However, challenges such as ammonia inhibition still remain. Quorum sensing (QS) system is relevant with the regulation of microbial community behaviors by releasing and sensing signal molecules, which could improve methane production during AD process. Therefore, the current study explored the effects of different quorum sensing signal molecules on alleviation of ammonia inhibition. The results showed that both secretion of N-butyryl-DL-homoserine lactone (C4-HSL) and N-(β-ketocaproyl)-DL-homoserine lactone (3OC6-HSL) could be inhibited by high ammonia stress while stimulation of N-hexanoyl-L-homoserine lactone (C6-HSL) and N-octanoyl-DL-homoserine lactone (C8-HSL) secretion might be triggered by ammonia toxicity. Moreover, the alleviation of ammonia inhibition could be achieved by both introducing 3OC6-HSL (0.5 μM) and combination of 3OC6-HSL (0.1 μM) and biochar (4 g/L). Exogenous 3OC6-HSL could regulate microbial social behaviors and enhance the secretion of extracellular polymeric substances (EPS) to promote anaerobic digestion. In addition, the mitigation of ammonia inhibition through exogenous 3OC6-HSL and biochar were confirmed by microbial community changes (Methanobacterium, Propionicicella and Petrimonas). Critical enzymes involved in both acidification and methanogenic steps were enhanced after adding the combination of 3OC6-HSL and biochar. The combination of low levels of 3OC6-HSL and biochar could promote both direct interspecies electron transfer (DIET) process and communication between different anaerobic microorganisms to mitigate ammonia inhibition. The current study will provide primary insights for conquering ammonia inhibition during biomethanation.
期刊介绍:
The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.