The antimicrobial properties of exogenous copper in human synovial fluid against Staphylococcus aureus.

IF 4.7 2区 医学 Q2 CELL & TISSUE ENGINEERING Bone & Joint Research Pub Date : 2024-11-07 DOI:10.1302/2046-3758.1311.BJR-2024-0148.R1
Fernando Diaz Dilernia, David Watson, David Heinrichs, Edward Vasarhelyi
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Abstract

Aims: The mechanism by which synovial fluid (SF) kills bacteria has not yet been elucidated, and a better understanding is needed. We sought to analyze the antimicrobial properties of exogenous copper in human SF against Staphylococcus aureus.

Methods: We performed in vitro growth and viability assays to determine the capability of S. aureus to survive in SF with the addition of 10 µM of copper. We determined the minimum bactericidal concentration of copper (MBC-Cu) and evaluated its sensitivity to killing, comparing wild type (WT) and CopAZB-deficient USA300 strains.

Results: UAMS-1 demonstrated a greater sensitivity to SF compared to USA300 WT at 12 hours (p = 0.001) and 24 hours (p = 0.027). UAMS-1 died in statistically significant quantities at 24 hours (p = 0.017), and USA300 WT survived at 24 hours. UAMS-1 was more susceptible to the addition of copper at four (p = 0.001), 12 (p = 0.005), and 24 hours (p = 0.006). We confirmed a high sensitivity to killing with the addition of exogenous copper on both strains at four (p = 0.011), 12 (p = 0.011), and 24 hours (p = 0.011). WT and CopAZB-deficient USA300 strains significantly died in SF, demonstrating a MBC-Cu of 50 µM against USA300 WT (p = 0.011).

Conclusion: SF has antimicrobial properties against S. aureus, and UAMS-1 was more sensitive than USA300 WT. Adding 10 µM of copper was highly toxic, confirming its bactericidal effect. We found CopAZB proteins to be involved in copper effluxion by demonstrating the high sensitivity of mutant strains to lower copper concentrations. Thus, we propose CopAZB proteins as potential targets and use exogenous copper as a treatment alternative against S. aureus.

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人体滑液中的外源性铜对金黄色葡萄球菌的抗菌特性。
目的:滑膜液(SF)杀灭细菌的机制尚未阐明,需要更好的理解。我们试图分析人体滑液中的外源性铜对金黄色葡萄球菌的抗菌特性:我们进行了体外生长和存活率测定,以确定金黄色葡萄球菌在添加 10 µM 铜的 SF 中存活的能力。我们测定了铜的最小杀菌浓度(MBC-Cu),并通过比较野生型(WT)和 CopAZB 缺陷的 USA300 菌株,评估了其杀菌敏感性:结果:与 USA300 WT 相比,UAMS-1 在 12 小时(p = 0.001)和 24 小时(p = 0.027)时对 SF 更敏感。在 24 小时内,UAMS-1 的死亡数量具有统计学意义(p = 0.017),而 USA300 WT 则在 24 小时内存活。UAMS-1 在 4 小时(p = 0.001)、12 小时(p = 0.005)和 24 小时(p = 0.006)时更容易受到铜的影响。我们证实,在 4 小时(p = 0.011)、12 小时(p = 0.011)和 24 小时(p = 0.011)时,两种菌株对添加外源铜的杀灭都很敏感。WT和CopAZB缺陷的USA300菌株在SF中明显死亡,对USA300 WT的MBC-Cu为50 µM(p = 0.011):结论:SF 对金黄色葡萄球菌具有抗菌特性,UAMS-1 比 USA300 WT 更敏感。加入 10 µM 的铜具有很强的毒性,证实了其杀菌作用。通过证明突变菌株对较低浓度铜的高度敏感性,我们发现 CopAZB 蛋白参与了铜的流出。因此,我们建议将 CopAZB 蛋白作为潜在靶标,利用外源铜作为治疗金黄色葡萄球菌的替代方法。
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来源期刊
Bone & Joint Research
Bone & Joint Research CELL & TISSUE ENGINEERING-ORTHOPEDICS
CiteScore
7.40
自引率
23.90%
发文量
156
审稿时长
12 weeks
期刊介绍: The gold open access journal for the musculoskeletal sciences. Included in PubMed and available in PubMed Central.
期刊最新文献
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