Comparison of the protective effects of silymarin and thymoquinone in the focal cerebral ischemia-reperfusion rat model.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-11-07 DOI:10.1080/10520295.2024.2421511

Merve Solmaz, Ender Erdogan, Dervis Dasdelen, Rasim Mogulkoc, Husamettin Vatansev, Fikret Akyurek, Hanefi Ozbek

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Abstract

Silymarin and thymoquinone exert neuroprotective effects, although their combined effects in focal cerebral ischemia/reperfusion (I/R) models are unknown. We compared the effect of silymarin and thymoquinone in an I/R rat model. Wistar rats were divided into five groups: SHAM, REP (I/R), SIR (200 mg/kg silymarin+I/R), TIR (3 mg/kg thymoquinone+I/R), and STIR (200 mg/kg silymarin+3-mg thymoquinone+I/R). The rats underwent bilateral carotid artery occlusion for 30 min and neurological assessments 24 h thereafter. Apoptosis was evaluated using anti-caspase-3 and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assays. Astrocyte activation was determined using an anti-GFAP antibody. Total antioxidant status (TAS), total oxidant status (TOS), and trimethylamine N-oxide (TMAO) levels were measured. SHAM and REP rats had the lowest and highest neurological scores, respectively (p = 0.001). REP rats showed greater deterioration than SIR, TIR, and STIR rats. SIR, TIR, and STIR rats had fewer TUNEL and caspase-3-positive cells than REP rats (p<0.05). GFAP expression was higher in REP rats (p<0.05) than in SIR, TIR, and STIR rats (p<0.05). SIR and TIR rats showed higher TAS than REP rats (p<0.05). SIR, TIR, and STIR rats had lower TMAO values than REP and SHAM rats (p<0.05). Silymarin/thymoquinone reduces impairment, apoptosis, and astrocyte activation. Combination therapy reduces TMAO levels.

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比较水飞蓟素和胸腺醌在局灶性脑缺血再灌注大鼠模型中的保护作用

水飞蓟素和胸腺醌具有神经保护作用，但它们在局灶性脑缺血/再灌注（I/R）模型中的联合作用尚不清楚。我们比较了水飞蓟素和胸腺醌在 I/R 大鼠模型中的作用。Wistar 大鼠被分为五组：SHAM组、REP组（I/R）、SIR组（200毫克/千克水飞蓟素+I/R）、TIR组（3毫克/千克胸腺醌+I/R）和STIR组（200毫克/千克水飞蓟素+3毫克胸腺醌+I/R）。大鼠接受双侧颈动脉闭塞 30 分钟，之后 24 小时接受神经系统评估。使用抗天冬酶-3和末端脱氧核苷酸转移酶生物素-DUTP缺口末端标记（TUNEL）检测法评估细胞凋亡。使用抗 GFAP 抗体测定星形胶质细胞的活化。对总抗氧化状态（TAS）、总氧化状态（TOS）和三甲胺 N-氧化物（TMAO）水平进行了测定。SHAM 和 REP 大鼠的神经评分分别最低和最高（p = 0.001）。与 SIR、TIR 和 STIR 大鼠相比，REP 大鼠的病情恶化程度更高。与 REP 大鼠相比，SIR、TIR 和 STIR 大鼠的 TUNEL 和 caspase-3 阳性细胞更少（p = 0.001）。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.