A Distinct Genotype, D/Ep6, Detected in Korean Female Patients: Chlamydia trachomatis Characterization from 2017 - 2018.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Clinical laboratory Pub Date : 2024-11-01 DOI:10.7754/Clin.Lab.2024.240609

Yeon-Joo Choi, Taeuk Kang, Kyoung Ho Roh, Won-Jong Jang

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Abstract

Background: The objective of this study lies in identifying the dominant genotype of C. trachomatis isolated in Republic of Korea (ROK) between 2017 and 2018.

Methods: A total of 504 clinical cervicovaginal swabs from patients were collected and inoculated on McCoy cell monolayers to isolate Chlamydial agents. C. trachomatis isolates were analyzed by sequencing of its ompA gene.

Results: A total of 54 C. trachomatis isolates were obtained. The constructed phylogenetic tree revealed the genotypes of isolates are D/Ep6 (48, 88.89%), D/Ep6-like (5, 9.26%), and Ja (1, 1.85%). The C. trachomatis D/Ep6-like have one single nucleotide polymorphism (SNP) compared to C. trachomatis D/Ep6, leading to E870K amino acid change.

Conclusions: Phylogenetic analysis demonstrated globally rare C. trachomatis D/Ep6 was dominant in the ROK from 2017 to 2018. Findings in this study act as a keystone, bridging past and present molecular epidemiology of C. trachomatis in context of ROK.

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在韩国女性患者中检测到一种独特的基因型 D/Ep62017-2018年沙眼衣原体特征。

背景：本研究的目的在于确定2017年至2018年间大韩民国（ROK）分离的沙眼衣原体的优势基因型：共收集了 504 份来自患者的临床宫颈阴道拭子，并接种到麦考伊细胞单层上以分离衣原体。通过对沙眼衣原体的 ompA 基因进行测序，对分离出的沙眼衣原体进行分析：结果：共获得 54 个沙眼衣原体分离株。构建的系统发生树显示分离株的基因型为 D/Ep6（48 株，88.89%）、D/Ep6-like（5 株，9.26%）和 Ja（1 株，1.85%）。与沙眼衣原体 D/Ep6 相比，沙眼衣原体 D/Ep6-like 有一个单核苷酸多态性（SNP），导致 E870K 氨基酸变化：系统发育分析表明，2017 年至 2018 年，全球罕见的沙眼衣原体 D/Ep6 在韩国占主导地位。本研究的发现是一个关键，它连接了过去和现在韩国沙眼衣原体的分子流行病学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.