Melanie Fisher, Bailey H Duhon, Han T N Nguyen, Jeffrey R Tonniges, Kyle C Wu, Yin Ren
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Abstract
Objective: The primary objective was to characterize the abundance and architecture of collagen in the extracellular matrix in vestibular schwannoma (VS). The secondary objective was to investigate the association between collagen architecture and tumor size.
Study design: Retrospective cohort study.
Setting: Academic referral center.
Methods: Tumor samples were obtained from patients with sporadic VS undergoing microsurgical resection. Histological analyses were performed including picrosirius red (PSR) staining under polarized light. Collagen architecture was quantified using an automated fiber detection software. Second Harmonic Generation (SHG) microscopy and immunofluorescence (IF) were utilized to characterize collagen architecture.
Results: Eleven tumor specimens were included (mean tumor diameter = 2.80 cm, range 1.5-4.0 cm), and were divided into large (mean diameter = 3.5 ± 0.4 cm) and small (mean tumor diameter = 2.0 ± 0.4 cm) cohorts based on size. The large VS cohort showed significantly higher collagen density (27.65% vs 12.73%, P = .0043), with more thick fibers (mature Type I, 24.54% vs 12.97%, P = .0022) and thin fibers (immature Type I or mature Type III, 23.55% vs 12.27%, P = .026). Tumor volume correlated with greater degree of collagen fiber disorganization (P = .0413, r2 = 0.298). Specifically, collagen type I intensity was significantly higher in large VS compared to small tumors (P < .001) and peripheral nerve (P = .028).
Conclusion: Larger VS exhibit increased collagen abundance in the tumor stroma, and a more disorganized collagen architecture compared to smaller VS and normal peripheral nerve tissue. This finding indicates that collagen organization may play a significant role in extracellular matrix remodeling and the progression of VS.
目的：主要目的是描述前庭裂隙瘤（VS）细胞外基质中胶原蛋白的丰度和结构。研究设计：回顾性队列研究：研究设计：回顾性队列研究：地点：学术转诊中心：肿瘤样本来自接受显微外科切除术的散发性 VS 患者。进行组织学分析，包括偏振光下的皮色红（PSR）染色。使用自动纤维检测软件对胶原结构进行量化。利用二次谐波发生（SHG）显微镜和免疫荧光（IF）来描述胶原结构：共纳入 11 例肿瘤标本（平均肿瘤直径 = 2.80 厘米，范围为 1.5-4.0 厘米），并根据大小分为大肿瘤（平均直径 = 3.5 ± 0.4 厘米）和小肿瘤（平均肿瘤直径 = 2.0 ± 0.4 厘米）。大型 VS 队列的胶原蛋白密度明显更高（27.65% vs 12.73%，P = .0043），有更多的粗纤维（成熟 I 型，24.54% vs 12.97%，P = .0022）和细纤维（未成熟 I 型或成熟 III 型，23.55% vs 12.27%，P = .026）。肿瘤体积与胶原纤维紊乱程度相关（P = .0413，r2 = 0.298）。具体来说，与小型肿瘤相比，大型 VS 的 I 型胶原蛋白强度明显更高（P 结论：大型 VS 的 I 型胶原蛋白强度明显高于小型肿瘤：与较小的 VS 和正常周围神经组织相比，较大的 VS 表现出肿瘤基质中胶原丰度增加，胶原结构更紊乱。这一发现表明，胶原组织可能在细胞外基质重塑和 VS 进展中发挥重要作用。
期刊介绍：
Otolaryngology–Head and Neck Surgery (OTO-HNS) is the official peer-reviewed publication of the American Academy of Otolaryngology–Head and Neck Surgery Foundation. The mission of Otolaryngology–Head and Neck Surgery is to publish contemporary, ethical, clinically relevant information in otolaryngology, head and neck surgery (ear, nose, throat, head, and neck disorders) that can be used by otolaryngologists, clinicians, scientists, and specialists to improve patient care and public health.
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