Reactive oxygen species-responsive polydopamine-PtCuTe nanoparticle-loaded microneedle system for promoting the healing of infected skin wounds

IF 10.5 1区 医学 Q1 CHEMISTRY, MULTIDISCIPLINARY Journal of Controlled Release Pub Date : 2024-11-07 DOI:10.1016/j.jconrel.2024.11.002
Hongfan Che , Junzhi Xu , Dong Wu , Siliang Chen , Chengkang Liu , Chongbao Zhao , Kun Peng
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Abstract

Nanozymes, known for their high efficiency in scavenging reactive oxygen species (ROS), have received significant attention in promoting the healing of infected wounds. Herein, we reported a novel multifunctional PDA-PtCuTe nanozyme with excellent ROS scavenging, antibacterial, pro-angiogenic, anti-inflammatory, and immune regulatory properties. It was loaded onto microneedles (PTPP-MN) for treating infected wounds. In vitro experiments demonstrated its ability to scavenge ROS and exhibit antioxidant properties. Compared to PT-MN (11.03 ± 3.37 %) and PTP-MN (42.30 ± 2.60 %), the ROS scavenging rate of PTPP-MN reached 63.63 ± 4.42 %. The microneedle exhibits good biocompatibility, stimulating fibroblast migration, endothelial angiogenesis, and M2 macrophage polarization. Additionally, it effectively eliminates ROS and provides antioxidant effects while inhibiting the viability of S. aureus and E. coli. Animal experiments showed that the PTPP-MN group achieved near-complete re-epithelialization by the third day compared to other groups. Histological observations revealed that the PTPP-MN group exhibited enhanced granulation tissue formation, epithelial regeneration, and angiogenesis. After PTPP-MN treatment, the local immune response shifted from a pro-inflammatory state to a pro-regenerative state. Our results indicate that PTPP-MN holds great promise for infected wound healing with reduced scar formation.

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促进感染性皮肤伤口愈合的活性氧反应型多巴胺-铂铜碲纳米粒子微针系统。
纳米酶以其高效清除活性氧(ROS)而闻名,在促进感染伤口愈合方面备受关注。在此,我们报告了一种新型多功能 PDA-PtCuTe 纳米酶,它具有出色的清除 ROS、抗菌、促血管生成、抗炎和免疫调节特性。它被载入微针(PTPP-MN),用于治疗感染伤口。体外实验证明了它清除 ROS 和抗氧化的能力。与 PT-MN(11.03 ± 3.37 %)和 PTP-MN(42.30 ± 2.60 %)相比,PTPP-MN 的 ROS 清除率达到了 63.63 ± 4.42 %。微针具有良好的生物相容性,可刺激成纤维细胞迁移、内皮血管生成和 M2 巨噬细胞极化。此外,它还能有效消除 ROS 并提供抗氧化作用,同时抑制金黄色葡萄球菌和大肠杆菌的活力。动物实验表明,与其他组相比,PTPP-MN 组在第三天就实现了几乎完全的再上皮化。组织学观察显示,PTPP-MN 组的肉芽组织形成、上皮再生和血管生成均有所增强。经 PTPP-MN 处理后,局部免疫反应从促炎症状态转变为促再生状态。我们的研究结果表明,PTPP-MN 在感染伤口愈合和减少疤痕形成方面大有可为。
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来源期刊
Journal of Controlled Release
Journal of Controlled Release 医学-化学综合
CiteScore
18.50
自引率
5.60%
发文量
700
审稿时长
39 days
期刊介绍: The Journal of Controlled Release (JCR) proudly serves as the Official Journal of the Controlled Release Society and the Japan Society of Drug Delivery System. Dedicated to the broad field of delivery science and technology, JCR publishes high-quality research articles covering drug delivery systems and all facets of formulations. This includes the physicochemical and biological properties of drugs, design and characterization of dosage forms, release mechanisms, in vivo testing, and formulation research and development across pharmaceutical, diagnostic, agricultural, environmental, cosmetic, and food industries. Priority is given to manuscripts that contribute to the fundamental understanding of principles or demonstrate the advantages of novel technologies in terms of safety and efficacy over current clinical standards. JCR strives to be a leading platform for advancements in delivery science and technology.
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