Fine mapping of QGPC.caas-7AL for grain protein content in bread wheat.

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-11-06 DOI:10.1007/s00122-024-04769-9
Dehui Zhao, Jianqi Zeng, Hui Jin, Dan Liu, Li Yang, Xianchun Xia, Yubing Tian, Yan Zhang, Shuanghe Cao, Wei Zhu, Chunping Wang, Zhonghu He, Jindong Liu, Yong Zhang
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Abstract

Key message: A major stable QTL, QGPC.caas-7AL, for grain protein content of wheat, was narrowed down to a 1.82-Mb inter on chromosome 7AL, and four candidate genes were predicated. Wheat grain protein content (GPC) is important for end-use quality. Identification of genetic loci for GPC is helpful to create new varieties with good processing quality and nutrients. Zhongmai 578 (ZM578) and Jimai 22 (JM22) are two elite wheat varieties with different contents of GPC. In the present study, 262 recombinant inbred lines (RILs) derived from a cross between ZM578 and JM22 were used to map the GPC with high-density wheat Illumina iSelect 50 K single-nucleotide polymorphism (SNP) array. Seven quantitative trait loci (QTLs) were identified for GPC on chromosomes 3AS, 3AL, 3BS, 4AL, 5BS, 5DL and 7AL by inclusive composite interval mapping, designated as QGPC.caas-3AS, QGPC.caas-3AL, QGPC.caas-3BS, QGPC.caas-4AL, QGPC.caas-5BS, QGPC.caas-5DL and QGPC.caas-7AL, respectively. Among these, alleles for increasing GPC at QGPC.caas-3AS, QGPC.caas-3BS, QGPC.caas-4AL and QGPC.caas-7AL loci were contributed by ZM578, whereas those at the other three loci were from JM22. The stable QTL QGPC.caas-7AL was fine mapped to a 1.82-Mb physical interval using secondary populations from six heterozygous recombinant plants obtained by selfing a residual RIL. Four genes were predicted as candidates of QGPC.caas-7AL based on sequence polymorphism and expression patterns. The near-isogenic lines (NILs) with the favorable allele at the QGPC.caas-7AL locus increased Farinograph stability time, Extensograph extension area, extensibility and maximum resistance by 19.6%, 6.3%, 6.0% and 20.3%, respectively. Kompetitive allele-specific PCR (KASP) marker for QGPC.caas-7AL was developed and validated in a diverse panel of 166 Chinese wheat cultivars. These results provide further insight into the genetic basis of GPC, and the fine-mapped QGPC.caas-7AL will be an attractive target for map-based cloning and marker-assisted selection in wheat breeding programs.

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QGPC.caas-7AL对面包小麦谷物蛋白质含量的精细测绘。
关键信息:小麦谷粒蛋白质含量的主要稳定QTL QGPC.caas-7AL被缩小到染色体7AL上的1.82-Mb区间,并确定了4个候选基因。小麦谷物蛋白质含量(GPC)对最终使用品质非常重要。鉴定 GPC 的基因位点有助于培育出加工品质好、营养成分高的新品种。中麦 578(ZM578)和济麦 22(JM22)是两个 GPC 含量不同的小麦优良品种。本研究利用 ZM578 和 JM22 杂交产生的 262 个重组近交系(RILs),通过高密度小麦 Illumina iSelect 50 K 单核苷酸多态性(SNP)阵列绘制了 GPC 图谱。通过包容性复合区间作图,在 3AS、3AL、3BS、4AL、5BS、5DL 和 7AL 染色体上鉴定出了 7 个 GPC 的数量性状位点(QTLs),分别命名为 QGPC.caas-3AS、QGPC.caas-3AL、QGPC.caas-3BS、QGPC.caas-4AL、QGPC.caas-5BS、QGPC.caas-5DL 和 QGPC.caas-7AL。其中,QGPC.caas-3AS、QGPC.caas-3BS、QGPC.caas-4AL和QGPC.caas-7AL位点的等位基因由ZM578贡献,而其他三个位点的等位基因来自JM22。利用从一株残留 RIL 自交获得的六株杂合重组植株的次级群体,将稳定 QTL QGPC.caas-7AL 精细映射到一个 1.82-Mb 的物理区间。根据序列多态性和表达模式,预测出四个基因为 QGPC.caas-7AL 的候选基因。QGPC.caas-7AL位点上有利等位基因的近等基因系(NIL)的法林图稳定时间、伸展面积、伸展性和最大抗性分别增加了19.6%、6.3%、6.0%和20.3%。开发了 QGPC.caas-7AL 的竞争性等位基因特异性 PCR(KASP)标记,并在 166 个中国小麦栽培品种的多样性面板中进行了验证。这些结果进一步揭示了GPC的遗传基础,精细图谱QGPC.caas-7AL将成为小麦育种计划中基于图谱克隆和标记辅助选择的一个有吸引力的目标。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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