Early Days in the Hunt Laboratory at UVA, 1969-1980.

IF 6.1 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS Molecular & Cellular Proteomics Pub Date : 2024-11-04 DOI:10.1016/j.mcpro.2024.100874
P Jane Gale, George C Stafford, Howard R Morris, Charles N McEwen
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Abstract

Arriving at the University of Virginia in the autumn of 1969, Donald Hunt began his 50+ year career in academics with the study of organometallic chemistry, on which he had done his PhD thesis, and mass spectrometry, to which he was introduced while a postdoc in Klaus Biemann's laboratory at the Massachusetts Institute of Technology. In the 1970s, Hunt's lab pioneered the use of negative chemical ionization (CI) to enhance sensitivity for studying organic molecules, developed a system for simultaneously obtaining positive and negative CI spectra to augment structure elucidation, and built a prototype triple quadrupole instrument so effective at collisional dissociation that its commercial counterpart became the analytical instrument of choice for mixture analysis for the next decade and beyond. Foreseeing that the future lay in the analysis of biological molecules, by the end of the decade Hunt shifted his focus to peptides. The analysis of protein fragments had suddenly become more accessible thanks to the advent of the triple quadrupole and Barber's invention of fast atom bombardment. As the '80s began and Hunt and his team sought to pursue larger and larger pieces of proteins, his attention turned to the development of mass spectrometers with greater mass range. While recounting their memories of these events, several of Hunt's students and colleagues pay tribute to his support for them as individuals, as well as to his infectious enthusiasm for scientific endeavors that he so generously shared.

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弗吉尼亚大学亨特实验室的早期生活,1969-1980 年。
1969 年秋天,唐纳德-亨特来到弗吉尼亚大学,开始了他长达 50 多年的学术生涯。他的博士论文研究方向是有机金属化学,而他在麻省理工学院克劳斯-比曼 (Klaus Biemann) 实验室做博士后时开始接触质谱分析。20 世纪 70 年代,亨特的实验室率先使用负化学电离 (CI) 技术来提高有机分子研究的灵敏度,开发了一套同时获得正负 CI 光谱的系统来增强结构阐释能力,并建立了一个三重四极杆仪器原型,该仪器在碰撞解离方面非常有效,以至于其商业同类仪器在接下来的十年中成为混合物分析的首选。由于预见到生物分子分析是未来的发展趋势,亨特在这十年的末尾将工作重点转移到了肽的分析上。由于三重四极杆的出现和巴伯发明的快速原子轰击技术,蛋白质片段的分析突然变得更加容易。进入 80 年代后,亨特和他的团队开始寻求更大的蛋白质片段,他的注意力转向了质量范围更大的质谱仪的开发。在讲述他们对这些事件的回忆时,亨特的几位学生和同事对亨特作为个人对他们的支持以及他慷慨分享的对科学事业的感染力表示敬意。
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来源期刊
Molecular & Cellular Proteomics
Molecular & Cellular Proteomics 生物-生化研究方法
CiteScore
11.50
自引率
4.30%
发文量
131
审稿时长
84 days
期刊介绍: The mission of MCP is to foster the development and applications of proteomics in both basic and translational research. MCP will publish manuscripts that report significant new biological or clinical discoveries underpinned by proteomic observations across all kingdoms of life. Manuscripts must define the biological roles played by the proteins investigated or their mechanisms of action. The journal also emphasizes articles that describe innovative new computational methods and technological advancements that will enable future discoveries. Manuscripts describing such approaches do not have to include a solution to a biological problem, but must demonstrate that the technology works as described, is reproducible and is appropriate to uncover yet unknown protein/proteome function or properties using relevant model systems or publicly available data. Scope: -Fundamental studies in biology, including integrative "omics" studies, that provide mechanistic insights -Novel experimental and computational technologies -Proteogenomic data integration and analysis that enable greater understanding of physiology and disease processes -Pathway and network analyses of signaling that focus on the roles of post-translational modifications -Studies of proteome dynamics and quality controls, and their roles in disease -Studies of evolutionary processes effecting proteome dynamics, quality and regulation -Chemical proteomics, including mechanisms of drug action -Proteomics of the immune system and antigen presentation/recognition -Microbiome proteomics, host-microbe and host-pathogen interactions, and their roles in health and disease -Clinical and translational studies of human diseases -Metabolomics to understand functional connections between genes, proteins and phenotypes
期刊最新文献
Early Days in the Hunt Laboratory at UVA, 1969-1980. Screening of cancer-specific biomarkers for hepatitis B-related hepatocellular carcinoma based on a proteome microarray. On the Hunt for the Histone Code. Functional analysis of MS-based proteomics data: from protein groups to networks. Targeted dynamic phospho-proteogenomic analysis of gastric cancer cells suggests host immunity provides survival benefit.
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