Quantitative proteomics reveal the protective effects of Qiang Jing decoction against oligoasthenospermia via modulating spermatogenesis related-proteins.
Desheng Wang, Haiwang Lu, Bin Bin, Siwei Lin, Jie Wang
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引用次数: 0
Abstract
Background: According to the current situation, the treatment of oligoasthenospermia with Western medicine is still full of challenges. Qiang Jing decoction (QJD) is used for treating male infertility as a traditional Chinese medicine (TCM) prescription. In this study. we will try to uncover the molecular mechanism of QJDs in treating oligoasthenospermia based on the proteomics technology.
Methods: In this study, sperm quality from oligoasthenospermia model, QJD treatment, and control rats were analyzed. Tandem mass tag (TMT) quantitative proteomics was used for the comparative analysis of protein abundance in rat testis of different treatment.
Results: The therapeutic effect of QJDs on spermatogenesis function by improving sperm quality was confirmed in the present experiment. In addition, our results showed that the majority of proteins related to spermatogenesis were significantly downregulated in oligoasthenospermia model rats compared to the control group, whereas these proteins' expression levels were recovered after the QJD treatment. In the meantime, immunohistochemistry was used to determine PI3K/AKT (phosphoinositide 3 kinase/serine-threonine kinase) signaling pathway-related protein expression profiles. In brief, we think QJD improved sperm density and quality by activating spermatogenesis-related protein expression and PI3K/AKT signaling pathway.
Conclusions: The results of this study may help to establish a foundation for further functional studies of key protein-mediated for improving sperm quality and alleviating oligoasthenospermia.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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