Comparative biochemical analysis of platelet-rich plasma-chitosan and platelet-rich fibrin-chitosan for treating tibial bone defects in rabbits.

IF 1.7 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary World Pub Date : 2024-09-01 Epub Date: 2024-09-13 DOI:10.14202/vetworld.2024.2036-2043
Sajjad Javaid, Hamad Bin Rashid, Ayesha Safdar, Mamoona Chaudhry
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Abstract

Background and aim: Fracture healing can cause serious complications, both preoperatively and postoperatively, including malunion or non-union. Biomaterials can enhance the fracture healing process. This study aimed to compare platelet-rich plasma (PRP)-chitosan and platelet-rich fibrin (PRF)-chitosan on the basis of biochemical parameters for fracture treatment in rabbits.

Materials and methods: This study involved 12 clinically healthy rabbits. After preparing PRP and PRF, a 3-mm bone defect was created in the tibia of each rabbit. The animals were divided randomly into two groups (A and B). Group A received PRP-Chitosan, and Group B received PRF-Chitosan. Bone healing was assessed using biochemical parameters (calcium [Ca], phosphorus [P], serum alkaline phosphatase [ALP], and osteocalcin [Ocn]) at 2-, 4-, 6-, and 8-week postoperatively. The data were compared using repeated-measures analysis of variance (p < 0.05) with Statistical Package for the Social Sciences statistical software.

Results: Group-wise comparison showed no significant differences (p > 0.05) between the groups, except for ALP levels, which were significantly higher in Group B than in Group A (p < 0.05). In the week-wise comparison, there was a significant difference between both groups, as Ca and ALP levels showed significant differences at all weeks postoperatively, whereas Ocn showed a significant difference at 2- and 4-week postoperatively (p < 0.05). However, there was no difference in P levels between the groups at any post-operative week (p > 0.05).

Conclusion: Both combinations enhanced bone regeneration. However, PRF-Chitosan is a better combination for bone repair than PRP-Chitosan. There were some limitations of this study, such as a small sample size, only male rabbits were used, and a lack of mechanical testing; these limitations should be addressed in future studies. The insights gained from the present study may open a new approach to the use of a combination of biomaterials for bone healing, which should be further investigated clinically and in other animal models as a future scope.

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富血小板血浆壳聚糖和富血小板纤维蛋白壳聚糖治疗兔子胫骨缺损的生化比较分析
背景和目的:骨折愈合可引起严重的并发症,包括术前和术后的愈合不良或不愈合。生物材料可促进骨折愈合过程。本研究旨在比较富血小板血浆(PRP)-壳聚糖和富血小板纤维蛋白(PRF)-壳聚糖用于兔子骨折治疗的生化指标:本研究涉及 12 只临床健康兔子。制备 PRP 和 PRF 后,在每只兔子的胫骨上创建一个 3 毫米的骨缺损。动物被随机分为两组(A 组和 B 组)。A 组接受 PRP-壳聚糖,B 组接受 PRF-壳聚糖。在术后 2、4、6 和 8 周,使用生化指标(钙[Ca]、磷[P]、血清碱性磷酸酶[ALP]和骨钙[Ocn])评估骨愈合情况。使用社会科学统计软件包对数据进行重复测量方差分析比较(P < 0.05):组间比较显示,除 ALP 水平 B 组明显高于 A 组(P < 0.05)外,各组间无明显差异(P > 0.05)。在周比较中,两组之间存在显著差异,Ca 和 ALP 水平在术后各周均有显著差异,而 Ocn 在术后 2 周和 4 周有显著差异(P < 0.05)。然而,术后各周各组间的 P 水平均无差异(P > 0.05):结论:两种组合都能促进骨再生。结论:两种组合都能促进骨再生,但 PRF-壳聚糖的骨修复效果优于 PRP-壳聚糖。本研究存在一些局限性,如样本量较小、仅使用雄性兔子以及缺乏机械测试;这些局限性应在今后的研究中加以解决。从本研究中获得的启示可能为使用生物材料组合促进骨愈合开辟了一条新途径,今后应在临床和其他动物模型中进一步研究。
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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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