[Association Between the Protein Expressions of MutS Homologs and Villin and the Clinicopathological Characteristics in 310 Colon Cancer Patients].

Q3 Medicine 四川大学学报(医学版) Pub Date : 2024-09-20 DOI:10.12182/20240960207

Fangfang Chang, Xiaoshu Hu, Yiyang Wen, Ping Li, Yun Huangfu, Fengjuan Zhang, Jing Tan, Xuexia Cao

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引用次数: 0

Abstract

Objective: To examine the relationship between the expressions of mismatch repair proteins, MutS homolog 2 (MSH2) and MutS homolog 6 (MSH6), and villin and the pathological features in patients with colon cancer.

Methods: A total of 310 cases of colon cancer patients who were treated at our hospital between January 2017 and September 2021 were selected. The diagnosis of colon cancer of all patients was verified by pathological evaluation. Immunohistochemistry was used to determine the protein expressions of MSH2, MSH6, and villin. The correlation between the expressions of MSH2, MSH6, and villin and the clinicopathological parameters in patients with colon cancer was analyzed accordingly. Multivariate logistic regression was used to analyze the correlation between the expressions of MSH2, MSH6, and villin and the clinicopathological parameters of colon cancer. Kaplan-Meier survival curve was used to compare the 2-year survival rates of colon cancer patients with different expression levels of the proteins.

Results: Among the 310 patients with colon cancer, the negative expression rates of MSH2, MSH6, and villin proteins in cancer tissues were 8.71% (27/310), 9.35% (29/310), and 46.13% (143/310), respectively. The negative expression rates of the three proteins in tissues adjacent to cancer were 3.23% (10/310), 4.19% (13/310), and 9.68% (30/310), respectively. The negative expression rates of the three proteins in cancer tissues were all higher than those in adjacent tissues (P<0.05). Regression analysis showed that the expression of MSH2 and MSH6 in cancer tissues was correlated with the age, the location of tumor lesions, tumor differentiation degree, and lymph node metastasis in colon cancer patients (P<0.05). The expression of villin in the cancer tissue is correlated with the depth of tumor infiltration, lymph node metastasis, distant metastasis, and clinical staging status in colon cancer patients (P<0.05). The 2-year survival rates of patients with negative expressions of MSH2 and MSH6 were 51.85% and 44.83%, respectively, which were lower than those of patients with positive expression of MSH2 and MSH6 (79.51% and 80.43%, P<0.05). Thirteen patients (4.1%) had negative expression of MSH2, MSH6, and villin (referred to as "triple negative expressions") in the cancer tissues, and their 2-year survival rate was 30.77%, which was lower than that of colon cancer patients who did not meet the criteria for triple negative expressions (79.12% [235/297], P<0.05).

Conclusion: The expressions of MSH2, MSH6, and villin are closely correlated with the pathological features of colon cancer patients. Evaluating the expression of the three proteins may assist in the clinical diagnosis, treatment, and prognosis evaluation of colon cancer.

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[310 例结肠癌患者中 MutS 同源物和 Villin 蛋白表达与临床病理特征的关系］

目的探讨错配修复蛋白MutS同源物2（MSH2）、MutS同源物6（MSH6）和绒毛蛋白的表达与结肠癌患者病理特征的关系：选取2017年1月至2021年9月期间在我院接受治疗的结肠癌患者共310例。方法：选取 2017 年 1 月至 2021 年 9 月期间在我院接受治疗的结肠癌患者共 310 例，所有患者的结肠癌诊断均通过病理评估验证。免疫组化法检测 MSH2、MSH6 和绒毛蛋白的蛋白表达。分析结肠癌患者 MSH2、MSH6 和绒毛蛋白的表达与临床病理参数之间的相关性。采用多变量逻辑回归分析 MSH2、MSH6 和绒毛蛋白的表达与结肠癌临床病理参数的相关性。采用 Kaplan-Meier 生存曲线比较了不同蛋白表达水平的结肠癌患者的 2 年生存率：在310名结肠癌患者中，MSH2、MSH6和绒毛蛋白在癌组织中的负表达率分别为8.71%（27/310）、9.35%（29/310）和46.13%（143/310）。这三种蛋白在癌症邻近组织中的负表达率分别为 3.23%（10/310）、4.19%（13/310）和 9.68%（30/310）。三种蛋白在癌组织中的负表达率均高于邻近组织（PPPPP结论：MSH2、MSH6 和 villin 的表达与结肠癌患者的病理特征密切相关。评估这三种蛋白的表达有助于结肠癌的临床诊断、治疗和预后评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

四川大学学报(医学版) Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

0.70

自引率

0.00%

发文量

8695

期刊介绍： "Journal of Sichuan University (Medical Edition)" is a comprehensive medical academic journal sponsored by Sichuan University, a higher education institution directly under the Ministry of Education of the People's Republic of China. It was founded in 1959 and was originally named "Journal of Sichuan Medical College". In 1986, it was renamed "Journal of West China University of Medical Sciences". In 2003, it was renamed "Journal of Sichuan University (Medical Edition)" (bimonthly). "Journal of Sichuan University (Medical Edition)" is a Chinese core journal and a Chinese authoritative academic journal (RCCSE). It is included in the retrieval systems such as China Science and Technology Papers and Citation Database (CSTPCD), China Science Citation Database (CSCD) (core version), Peking University Library's "Overview of Chinese Core Journals", the U.S. "Index Medica" (IM/Medline), the U.S. "PubMed Central" (PMC), the U.S. "Biological Abstracts" (BA), the U.S. "Chemical Abstracts" (CA), the U.S. EBSCO, the Netherlands "Abstracts and Citation Database" (Scopus), the Japan Science and Technology Agency Database (JST), the Russian "Abstract Magazine", the Chinese Biomedical Literature CD-ROM Database (CBMdisc), the Chinese Biomedical Periodical Literature Database (CMCC), the China Academic Journal Network Full-text Database (CNKI), the Chinese Academic Journal (CD-ROM Edition), and the Wanfang Data-Digital Journal Group.