Back flux during anaerobic oxidation of butane support archaea-mediated alkanogenesis

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Nature Communications Pub Date : 2024-11-07 DOI:10.1038/s41467-024-53932-9

Song-Can Chen, Sheng Chen, Niculina Musat, Steffen Kümmel, Jiaheng Ji, Marie Braad Lund, Alexis Gilbert, Oliver J. Lechtenfeld, Hans-Hermann Richnow, Florin Musat

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Abstract

Microbial formation and oxidation of volatile alkanes in anoxic environments significantly impacts biogeochemical cycles on Earth. The discovery of archaea oxidizing volatile alkanes via deeply branching methyl-coenzyme M reductase variants, dubbed alkyl-CoM reductases (ACR), prompted the hypothesis of archaea-catalysed alkane formation in nature (alkanogenesis). A combination of metabolic modelling, anaerobic physiology assays, and isotope labeling of Candidatus Syntrophoarchaeum archaea catalyzing the anaerobic oxidation of butane (AOB) show a back flux of CO₂ to butane, demonstrating reversibility of the entire AOB pathway. Back fluxes correlate with thermodynamics and kinetics of the archaeal catabolic system. AOB reversibility supports a biological formation of butane, and generally of higher volatile alkanes, helping to explain the presence of isotopically light alkanes and deeply branching ACR genes in sedimentary basins isolated from gas reservoirs.

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丁烷厌氧氧化过程中的回流支持古细菌介导的产碱作用

缺氧环境中挥发性烷烃的微生物形成和氧化对地球上的生物地球化学循环有重大影响。古细菌通过被称为烷基-辅酶 M 还原酶（ACR）的深分支甲基辅酶 M 还原酶变体氧化挥发性烷烃的发现，引发了古细菌催化自然界烷烃形成（烷烃生成）的假设。通过对催化丁烷厌氧氧化（AOB）的合成古细菌进行代谢建模、厌氧生理学测定和同位素标记等综合研究，发现二氧化碳向丁烷的回流，证明了整个 AOB 途径的可逆性。逆通量与古细菌分解系统的热力学和动力学相关。AOB的可逆性支持丁烷的生物形成，一般也支持较高挥发性烷烃的生物形成，这有助于解释与天然气储层隔绝的沉积盆地中存在同位素轻烷烃和深分支ACR基因的原因。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.