Larvicidal and antiplasmodial studies of Eucalyptus camaldulensis (Myrtaceae) Leaf

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Advances in Traditional Medicine Pub Date : 2024-06-18 DOI:10.1007/s13596-024-00765-y
Stephen A. Adesida, Samuel A. Oguntimehin, Funmilayo G. Famuyiwa, Kolade O. Faloye, Seun B. Ogundele, Oyenike I. Bello, Oluyemi J. Oladiran, Ayobami J. Olusola, Adetola H. Adewole, Praise A. Adebayo, Maryam O. Oredola
{"title":"Larvicidal and antiplasmodial studies of Eucalyptus camaldulensis (Myrtaceae) Leaf","authors":"Stephen A. Adesida,&nbsp;Samuel A. Oguntimehin,&nbsp;Funmilayo G. Famuyiwa,&nbsp;Kolade O. Faloye,&nbsp;Seun B. Ogundele,&nbsp;Oyenike I. Bello,&nbsp;Oluyemi J. Oladiran,&nbsp;Ayobami J. Olusola,&nbsp;Adetola H. Adewole,&nbsp;Praise A. Adebayo,&nbsp;Maryam O. Oredola","doi":"10.1007/s13596-024-00765-y","DOIUrl":null,"url":null,"abstract":"<div><p><i>Eucalyptus camaldulensis</i> is a medicinal plant used as insect repellant and antimalarial agent in ethnomedicine. This study examined the antiplasmodial and larvicidal potential of <i>E. camaldulensis</i> leaf extract and also identified the plant extract's most active fraction(s). The acute oral toxicity test of the methanol extract was evaluated using Lorke’s method. The larvicidal assay was performed on the extract and partitioned fractions according to the 2005 World Health Organization guidelines, while the 4-day chemosuppressive and curative antimalarial assays were carried out against <i>Plasmodium bergei bergei</i>. Endosulphan and chloroquine (10 mg/kg) was used as the positive controls for the larvicidal and antiplasmodial assays, respectively, while tween 80 in normal saline (1%) was the negative control. The methanol leaf extract of EC showed good larvicidal activity across all tested concentrations (LC<sub>50</sub> 3.79 ± 0.64 mg/mL), while the aqueous fraction with LC<sub>50</sub> of 2.80 ± 0.14 mg/mL was the most active. Its acute toxicity test showed it was safe up to 5000 mg/kg. The extract significantly increased dose-dependent antiplasmodial activity for the chemosuppressive and curative models (<i>p</i> &lt; 0.05) than the negative control. At 800 mg/kg, EC gave chemosuppressive (53.69 ± 1.62%) and curative (81.26 ± 1.87%) activities, which was significantly lower than that of 10 mg/kg chloroquine (82.00 ± 0.57% and 92.51 ± 0.22% respectively). The aqueous partitioned fraction gave the highest chemosuppression (73.84 ± 2.73%) at 80 mg/kg, which was comparable to the positive control. The methanol extract of <i>E. camaldulensis</i> leaf is a promising larvicidal and antimalarial agent that could proffer a solution to vector control and the prevalence of malaria.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-024-00765-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0

Abstract

Eucalyptus camaldulensis is a medicinal plant used as insect repellant and antimalarial agent in ethnomedicine. This study examined the antiplasmodial and larvicidal potential of E. camaldulensis leaf extract and also identified the plant extract's most active fraction(s). The acute oral toxicity test of the methanol extract was evaluated using Lorke’s method. The larvicidal assay was performed on the extract and partitioned fractions according to the 2005 World Health Organization guidelines, while the 4-day chemosuppressive and curative antimalarial assays were carried out against Plasmodium bergei bergei. Endosulphan and chloroquine (10 mg/kg) was used as the positive controls for the larvicidal and antiplasmodial assays, respectively, while tween 80 in normal saline (1%) was the negative control. The methanol leaf extract of EC showed good larvicidal activity across all tested concentrations (LC50 3.79 ± 0.64 mg/mL), while the aqueous fraction with LC50 of 2.80 ± 0.14 mg/mL was the most active. Its acute toxicity test showed it was safe up to 5000 mg/kg. The extract significantly increased dose-dependent antiplasmodial activity for the chemosuppressive and curative models (p < 0.05) than the negative control. At 800 mg/kg, EC gave chemosuppressive (53.69 ± 1.62%) and curative (81.26 ± 1.87%) activities, which was significantly lower than that of 10 mg/kg chloroquine (82.00 ± 0.57% and 92.51 ± 0.22% respectively). The aqueous partitioned fraction gave the highest chemosuppression (73.84 ± 2.73%) at 80 mg/kg, which was comparable to the positive control. The methanol extract of E. camaldulensis leaf is a promising larvicidal and antimalarial agent that could proffer a solution to vector control and the prevalence of malaria.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
桉树(桃金娘科)叶的杀幼虫剂和抗疟研究
桉树是一种药用植物,在民族医药中被用作驱虫剂和抗疟药。本研究考察了喀麦隆桉叶提取物的抗疟和杀幼虫剂潜力,并确定了该植物提取物中最具活性的部分。采用 Lorke 法评估了甲醇提取物的急性口服毒性试验。根据 2005 年世界卫生组织指南,对萃取物和分配馏分进行了杀幼虫试验,并对贝氏疟原虫进行了为期 4 天的化学抑制和治疗性抗疟试验。杀幼虫剂和抗疟药物试验分别使用内吸磷和氯喹(10 毫克/千克)作为阳性对照,而正常生理盐水(1%)中的吐温 80 则作为阴性对照。氨基甲酸乙酯的甲醇叶提取物在所有测试浓度下都显示出良好的杀幼虫活性(半数致死浓度为 3.79 ± 0.64 毫克/毫升),而水提取物的半数致死浓度为 2.80 ± 0.14 毫克/毫升,活性最高。急性毒性测试表明,该提取物在 5000 毫克/千克以下是安全的。与阴性对照组相比,该提取物在化学抑制和治疗模型中的剂量依赖性抗疟活性明显提高(p < 0.05)。在 800 毫克/千克的剂量下,氨基甲酸乙酯具有化学抑制(53.69 ± 1.62%)和治疗(81.26 ± 1.87%)活性,明显低于 10 毫克/千克氯喹(分别为 82.00 ± 0.57% 和 92.51 ± 0.22%)。在 80 毫克/千克的剂量下,水分配馏分的化学抑制率最高(73.84 ± 2.73%),与阳性对照组相当。E.camaldulensis叶片的甲醇提取物是一种很有前景的杀幼虫剂和抗疟药剂,可为病媒控制和疟疾流行提供解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
期刊最新文献
In-vitro and in-silico evaluation of anticancer potential of Oxalis corniculata Effect of a traditional herbal formulation (Azaraghi Majoon) on opium use disorder in male rats Phyto-chemical, analytical evaluation and biological attributes of rare medicinal species of flowering shrub Bauhinia racemosa L. Validation of the constitution in Chinese medicine questionnaires with the newly developed blood-vacuity category: a rare Taiwanese case study Correction to: Unveiling the potential antibacterial mechanism of Melaleuca cajuputi leaf extract by cell morphology studies and molecular docking analysis
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1