Larvicidal and antiplasmodial studies of Eucalyptus camaldulensis (Myrtaceae) Leaf

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Advances in Traditional Medicine Pub Date : 2024-06-18 DOI:10.1007/s13596-024-00765-y
Stephen A. Adesida, Samuel A. Oguntimehin, Funmilayo G. Famuyiwa, Kolade O. Faloye, Seun B. Ogundele, Oyenike I. Bello, Oluyemi J. Oladiran, Ayobami J. Olusola, Adetola H. Adewole, Praise A. Adebayo, Maryam O. Oredola
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Abstract

Eucalyptus camaldulensis is a medicinal plant used as insect repellant and antimalarial agent in ethnomedicine. This study examined the antiplasmodial and larvicidal potential of E. camaldulensis leaf extract and also identified the plant extract's most active fraction(s). The acute oral toxicity test of the methanol extract was evaluated using Lorke’s method. The larvicidal assay was performed on the extract and partitioned fractions according to the 2005 World Health Organization guidelines, while the 4-day chemosuppressive and curative antimalarial assays were carried out against Plasmodium bergei bergei. Endosulphan and chloroquine (10 mg/kg) was used as the positive controls for the larvicidal and antiplasmodial assays, respectively, while tween 80 in normal saline (1%) was the negative control. The methanol leaf extract of EC showed good larvicidal activity across all tested concentrations (LC50 3.79 ± 0.64 mg/mL), while the aqueous fraction with LC50 of 2.80 ± 0.14 mg/mL was the most active. Its acute toxicity test showed it was safe up to 5000 mg/kg. The extract significantly increased dose-dependent antiplasmodial activity for the chemosuppressive and curative models (p < 0.05) than the negative control. At 800 mg/kg, EC gave chemosuppressive (53.69 ± 1.62%) and curative (81.26 ± 1.87%) activities, which was significantly lower than that of 10 mg/kg chloroquine (82.00 ± 0.57% and 92.51 ± 0.22% respectively). The aqueous partitioned fraction gave the highest chemosuppression (73.84 ± 2.73%) at 80 mg/kg, which was comparable to the positive control. The methanol extract of E. camaldulensis leaf is a promising larvicidal and antimalarial agent that could proffer a solution to vector control and the prevalence of malaria.

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桉树(桃金娘科)叶的杀幼虫剂和抗疟研究
桉树是一种药用植物,在民族医药中被用作驱虫剂和抗疟药。本研究考察了喀麦隆桉叶提取物的抗疟和杀幼虫剂潜力,并确定了该植物提取物中最具活性的部分。采用 Lorke 法评估了甲醇提取物的急性口服毒性试验。根据 2005 年世界卫生组织指南,对萃取物和分配馏分进行了杀幼虫试验,并对贝氏疟原虫进行了为期 4 天的化学抑制和治疗性抗疟试验。杀幼虫剂和抗疟药物试验分别使用内吸磷和氯喹(10 毫克/千克)作为阳性对照,而正常生理盐水(1%)中的吐温 80 则作为阴性对照。氨基甲酸乙酯的甲醇叶提取物在所有测试浓度下都显示出良好的杀幼虫活性(半数致死浓度为 3.79 ± 0.64 毫克/毫升),而水提取物的半数致死浓度为 2.80 ± 0.14 毫克/毫升,活性最高。急性毒性测试表明,该提取物在 5000 毫克/千克以下是安全的。与阴性对照组相比,该提取物在化学抑制和治疗模型中的剂量依赖性抗疟活性明显提高(p < 0.05)。在 800 毫克/千克的剂量下,氨基甲酸乙酯具有化学抑制(53.69 ± 1.62%)和治疗(81.26 ± 1.87%)活性,明显低于 10 毫克/千克氯喹(分别为 82.00 ± 0.57% 和 92.51 ± 0.22%)。在 80 毫克/千克的剂量下,水分配馏分的化学抑制率最高(73.84 ± 2.73%),与阳性对照组相当。E.camaldulensis叶片的甲醇提取物是一种很有前景的杀幼虫剂和抗疟药剂,可为病媒控制和疟疾流行提供解决方案。
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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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