New role of calcium-binding fluorescent dye alizarin complexone in detecting permeability from articular cartilage to subchondral bone

IF 2.5 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY FASEB bioAdvances Pub Date : 2024-10-07 DOI:10.1096/fba.2024-00103
Mingshu Cui, Mengcun Chen, Yanmei Yang, Hamza Akel, Bin Wang
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Abstract

Osteoarthritis (OA) is a chronic degenerative joint disorder characterized by the progressive deterioration of articular cartilage and concomitant alterations in subchondral bone architecture. However, the precise mechanisms underlying the initiation and progression of OA remains poorly understood. In the present study, we explored whether the calcification in the articular cartilage occurred in the early stage of mouse OA model, generated by the surgery destabilization of the medial meniscus (DMM), via the intra-articular injection of alizarin complexone due to its anionic nature for binding calcium-containing crystals. Although we did not observe the calcification in the articular cartilage of early stage of DMM mice, we unexpectedly identified alizarin complexone had the diffusion capacity for detecting the permeability from the articular cartilage to subchondral bone. Our data showed that the diffusion of alizarin complexone from the articular cartilage to calcified cartilage was greater in the early stage of DMM mice than that in sham controls. Additionally, we observed enhanced penetration of alizarin complexone through the periosteum in DMM mice compared to sham mice. In summary, we developed a novel imaging method that offers a valuable tool for further exploration of biochemical communication underlying OA development. Our findings provided new evidence that increased molecular interactions between the articular cartilage and subchondral bone is involved in the pathogenesis of OA progression.

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钙结合荧光染料茜素络合酮在检测从关节软骨到软骨下骨的渗透性方面的新作用
骨关节炎(OA)是一种慢性退行性关节疾病,其特点是关节软骨逐渐退化,同时软骨下骨结构发生改变。然而,人们对 OA 发生和发展的确切机制仍然知之甚少。在本研究中,我们通过向小鼠关节内注射茜素络合酮(alizarin complexone),探讨了小鼠 OA 模型的早期阶段,即通过手术破坏内侧半月板(DMM)而产生的关节软骨钙化是否发生,因为茜素络合酮具有结合含钙晶体的阴离子特性。虽然我们没有观察到早期 DMM 小鼠关节软骨中的钙化现象,但我们意外地发现茜素络合酮具有扩散能力,可以检测从关节软骨到软骨下骨的渗透性。我们的数据显示,与假对照组相比,DMM 早期小鼠的茜素络合酮从关节软骨向钙化软骨的扩散量更大。此外,与假对照组相比,我们还观察到茜素络合酮在 DMM 小鼠骨膜中的穿透力增强。总之,我们开发了一种新的成像方法,为进一步探索 OA 发病背后的生化通讯提供了一种宝贵的工具。我们的研究结果提供了新的证据,证明关节软骨和软骨下骨之间的分子相互作用增加参与了 OA 进展的发病机制。
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来源期刊
FASEB bioAdvances
FASEB bioAdvances Multiple-
CiteScore
5.40
自引率
3.70%
发文量
56
审稿时长
10 weeks
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