Detection of Salmonella enterica in food using targeted mass spectrometry

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-11-09 DOI:10.1016/j.foodchem.2024.141985
Mengqi Chen, Miaoxi Peng, Muyun Yuan, Chengdong Huang, Jingwen Liu, Zuqing Wu, Wenrui Chen, Songqing Hu, Qing Liu, Jie Dong, Li Ling
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Abstract

The high prevalence of Salmonella enterica necessitates rapid and efficient detection methods. Targeted mass spectrometry (MS) using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) has become a promising technique with improved specificity and sensitivity. We develop a novel targeted MS method for detecting S. enterica in food based on peptide biomarkers. Using a combination of four peptide biomarkers, this newly developed method could accurately distinguish S. enterica from other conventional food-borne pathogens. When combined with buoyant density centrifugation (BDC), Salmonella was efficiently separated from food matrices. Based on this discovery, this method was successfully applied to detect S. enterica in both artificially and naturally contaminated food samples, comparable to the culture method. These results demonstrate the potential of the targeted MS method in various food categories and are expected to be an alternative approach for S. enterica detection in food.
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利用靶向质谱法检测食品中的肠炎沙门氏菌
肠炎沙门氏菌的高流行率需要快速高效的检测方法。使用多反应监测(MRM)和平行反应监测(PRM)的靶向质谱(MS)技术具有更高的特异性和灵敏度,已成为一种很有前途的技术。我们开发了一种基于多肽生物标记物的新型靶向质谱方法,用于检测食品中的肠道病毒。利用四种肽生物标记物的组合,这种新开发的方法可以准确地区分肠炎双球菌和其他传统的食源性病原体。当与浮力密度离心法(BDC)结合使用时,沙门氏菌可从食物基质中有效分离出来。基于这一发现,该方法被成功应用于检测人工和自然污染的食品样本中的肠炎沙门氏菌,效果与培养法相当。这些结果证明了靶向质谱法在各类食品中的应用潜力,有望成为检测食品中肠道病毒的另一种方法。
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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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