Aggregation-induced emission (AIE) of Au(I)-GSH complexes activated by cationic polymer for sensitive foodborne pathogens detection and inactivation

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-11-09 DOI:10.1016/j.foodchem.2024.141988

Ruimeng Sun, Jiayu Guo, Yurou Wang, Han Wang, Haoran Zheng, Yanfei Qi

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Abstract

Aggregation-induced emission (AIE) is a novel signal output method but is limited in pathogen sensing. Herein, a multifunctional biosensor based on the AIE properties of Au(I)-GSH complexes as signal conversion tags was firstly constructed for rapid and sensitive total bacteria. Bacteria were captured by the boronic acid group of MNPs@Au@4-MPBA (MAu@MPBA) through recognition of peptidoglycan on their surface. Simultaneously, cationic polymer Poly (diallyldimethylammonium chloride) (PDDA) were electrostatic absorb on bacteria. After magnetic separation, the remaining PDDA induced Au(I)-GSH complexes aggregation to produce strong red fluorescence, which was linearly with the quantity of bacteria. Under optimized conditions, quantitative detection of bacteria can be achieved within 60 min, with a minimum detection concentration of 18 CFU/mL. Moreover, 90 % bacteria can be effectively inactivated while being detected. This strategy is capable of sensitively detecting and killing foodborne pathogens and can be successfully applied to food safety monitoring.

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阳离子聚合物激活的 Au(I)-GSH 复合物的聚集诱导发射 (AIE)，用于灵敏检测和灭活食源性病原体

聚集诱导发射（AIE）是一种新颖的信号输出方法，但在病原体传感方面存在局限性。本文首次构建了一种基于 Au（I）-GSH 复合物的 AIE 特性的多功能生物传感器，作为信号转换标签，用于快速灵敏地检测细菌总数。通过识别细菌表面的肽聚糖，MNPs@Au@4-MPBA（MAu@MPBA）的硼酸基团捕获了细菌。同时，阳离子聚合物聚（二烯丙基二甲基氯化铵）（PDDA）静电吸附细菌。磁分离后，剩余的 PDDA 诱导 Au(I)-GSH 复合物聚集，产生强烈的红色荧光，荧光与细菌数量呈线性关系。在优化条件下，细菌的定量检测可在 60 分钟内完成，最低检测浓度为 18 CFU/mL。此外，90% 的细菌可在检测过程中被有效灭活。这种策略能够灵敏地检测和杀死食源性病原体，可成功应用于食品安全监控。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture