Neopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum

IF 3.1 3区化学 Q2 POLYMER SCIENCE Polymer Bulletin Pub Date : 2024-09-25 DOI:10.1007/s00289-024-05516-2

Burcu Eren Yüngeviş, Gözde Baydemir Peşint

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Abstract

Neopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5° to 45.87° and 32.7° for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.

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蝶呤印迹纳米薄膜装饰的 SPR 传感器：从人体血清中灵敏检测蝶呤

蝶呤（Np）是监测传染病和预测预后的重要标志物。本研究开发了 Np 压印表面等离子体共振 (SPR) 传感器，专门用于检测人血清中的 Np。使用各种技术对 Np 压印（Np-MIP）和非压印（NIP）SPR 传感器进行了表征。接触角测量显示，纳米薄膜覆盖后，金传感器的亲水性增强，NIP 和 Np-MIP SPR 传感器的接触角分别从 60.5° 减小到 45.87° 和 32.7°。通过原子力显微镜研究确定纳米薄膜厚度约为 14 纳米。在不同浓度下，Np-MIP SPR 传感器成功实现了对 Np 的实时检测。通过对蝶呤和葡萄糖的测试，证实了 Np-MIP 的选择性，其 Np 识别率分别是蝶呤和葡萄糖的 11 倍和 8.25 倍。从人体血清中检测 Np 的检测限为 3 纳克/毫升。此外，合成的 Np-MIP SPR 传感器可重复使用 10 个周期，而 Np 结合能力没有任何下降。这些研究结果表明，Np-MIP SPR 传感器对水溶液和人体血清中的 Np 具有选择性检测能力。

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来源期刊

Polymer Bulletin 化学-高分子科学

CiteScore

6.00

自引率

6.20%

发文量

审稿时长

5.5 months

期刊介绍： "Polymer Bulletin" is a comprehensive academic journal on polymer science founded in 1988. It was founded under the initiative of the late Mr. Wang Baoren, a famous Chinese chemist and educator. This journal is co-sponsored by the Chinese Chemical Society, the Institute of Chemistry, and the Chinese Academy of Sciences and is supervised by the China Association for Science and Technology. It is a core journal and is publicly distributed at home and abroad. "Polymer Bulletin" is a monthly magazine with multiple columns, including a project application guide, outlook, review, research papers, highlight reviews, polymer education and teaching, information sharing, interviews, polymer science popularization, etc. The journal is included in the CSCD Chinese Science Citation Database. It serves as the source journal for Chinese scientific and technological paper statistics and the source journal of Peking University's "Overview of Chinese Core Journals."