Carboxymethyl cellulose-induced Cryptococcus laurentii improves disease resistance and regulates phenylpropane and reactive oxygen metabolism in grapefruit

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-11-12 DOI:10.1016/j.foodchem.2024.141955

Le Yang, Diantong Ma, Fang Wang, Li Liu, Lin Chen, Xiahong He, Junrong Tang, Jia Deng

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Abstract

Green mould disease poses a significant threat to the citrus industry. Cryptococcus laurentii can stimulate the fruit defence system, whereas the use of antagonistic yeast alone demonstrates limited efficacy. This study investigated the molecular mechanisms of C. laurentii cultured with carboxymethyl cellulose (CMC Abstract Image

C. laurentii), and evaluated the effects of CMC Abstract Image

C. laurentii on phenylpropane and reactive oxygen metabolism in grapefruit fruit. Transcriptome analysis revealed that the upregulation of gene expression associated with yeast growth and antagonistic ability occurred in CMC Abstract Image

C. laurentii after 72 h cultivation. Meanwhile, CMC Abstract Image

C. laurentii reduced lesion diameter and disease incidence in fruit. This treatment promoted phenylpropane metabolism by activating PAL, C4H, 4CL, POD, and PPO and increasing the secondary metabolites. CMC Abstract Image

C. laurentii also activated the AsA-GSH cycle, enhanced the activities of SOD and CAT, and reduced the accumulation of H₂O₂ and O₂^•-. The results suggested that CMC Abstract Image

C. laurentii maintained high postharvest fruit quality in grapefruit fruit by elevating the phenylpropane and reactive oxygen metabolism.

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羧甲基纤维素诱导的月桂隐球菌可提高葡萄柚的抗病性，并调节苯丙氨酸和活性氧代谢

绿霉病对柑橘产业构成了重大威胁。月桂隐球菌可以刺激果实的防御系统，而单独使用拮抗酵母菌的效果有限。本研究调查了用羧甲基纤维素培养的月桂隐球菌（CMCC.laurentii）的分子机制，并评估了 CMCC.laurentii 对葡萄柚果实中苯丙烷和活性氧代谢的影响。转录组分析表明，经过 72 小时的培养，CMCC.laurentii 中与酵母生长和拮抗能力相关的基因表达出现上调。同时，CMCC.laurentii 降低了果实的病变直径和病害发生率。该处理通过激活 PAL、C4H、4CL、POD 和 PPO 以及增加次生代谢产物来促进苯丙氨酸代谢。月桂酵母菌还能激活 AsA-GSH 循环，提高 SOD 和 CAT 的活性，减少 H2O2 和 O2 的积累。结果表明，CMCC.laurentii 通过提高苯丙氨酸和活性氧代谢，保持了葡萄柚果实采后的高品质。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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