Qunxiang Ong, Ler Ting Rachel Lim, Cameron Goh, Yilie Liao, Sher En Chan, Crystal Jing Yi Lim, Valerie Kam, Jerome Yap, Tiffany Tseng, Reina Desrouleaux, Loo Chien Wang, Siok Ghee Ler, Siew Lan Lim, Sun-Yee Kim, Radoslaw M. Sobota, Anton M. Bennett, Weiping Han, Xiaoyong Yang
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引用次数: 0
Abstract
The post-translational modification of intracellular proteins through O-linked β-N-acetylglucosamine (O-GlcNAc) is a conserved regulatory mechanism in multicellular organisms. Catalyzed by O-GlcNAc transferase (OGT), this dynamic modification has an essential role in signal transduction, gene expression, organelle function and systemic physiology. Here, we present Opto-OGT, an optogenetic probe that allows for precise spatiotemporal control of OGT activity through light stimulation. By fusing a photosensitive cryptochrome protein to OGT, Opto-OGT can be robustly and reversibly activated with high temporal resolution by blue light and exhibits minimal background activity without illumination. Transient activation of Opto-OGT results in mTORC activation and AMPK suppression, which recapitulate nutrient-sensing signaling. Furthermore, Opto-OGT can be customized to localize to specific subcellular sites. By targeting OGT to the plasma membrane, we demonstrate the downregulation of site-specific AKT phosphorylation and signaling outputs in response to insulin stimulation. Thus, Opto-OGT is a powerful tool for defining the role of O-GlcNAcylation in cell signaling and physiology.
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