A sandwich electrochemical immunosensor based on polypyrrole microspheres for the detection of the cancer marker CA125†

IF 2.6 3区化学 Q2 CHEMISTRY, ANALYTICAL Analytical Methods Pub Date : 2024-11-02 DOI:10.1039/D4AY01890G

Yan Ma, Tingting Zhang and Yuzhong Zhang

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Abstract

In this study, a sensitive sandwich electrochemical immunosensor for the detection of the cancer marker cancer antigen 125 (CA125) was developed using polypyrrole (PPy) microspheres for amplifying the sensing signal. The PPy microspheres provided a larger surface area for gold nanoparticle (Au NP), CA125 antibody and signal molecule adriamycin immobilization. In 0.10 M PBS of pH 7.4, a well-shaped oxidation peak for adriamycin at −0.83 V (vs. SCE) was obtained in a differential pulse voltammogram. Signal intensity was related to the level of CA125. Under optimal conditions, the immunosensor showed a wide linear response range of 10–100 U mL⁻¹ with a detection limit of 2.4 μU mL⁻¹ (at 3σ/N), and recovery was 98.1–101.2%, as evaluated using human serum samples. Furthermore, the immunosensor was applied to measure real serum samples, and the results obtained were in accordance with those of enzyme-linked immunosorbent assay (ELISA), indicating that the proposed immunosensor is an ideal platform for clinical diagnosis application.

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基于聚吡咯微球的夹心电化学免疫传感器，用于检测癌症标志物 CA125。

本研究利用聚吡咯（PPy）微球放大传感信号，开发了一种用于检测癌症标记物癌症抗原 125（CA125）的灵敏夹层电化学免疫传感器。PPy 微球为金纳米粒子（Au NP）、CA125 抗体和信号分子阿霉素的固定提供了更大的表面积。在 pH 值为 7.4 的 0.10 M PBS 中，阿霉素在差分脉冲伏安图中的-0.83 V（与 SCE 对比）处出现了一个形状清晰的氧化峰。信号强度与 CA125 的水平有关。在最佳条件下，该免疫传感器的线性响应范围为 10-100 U mL-1，检测限为 2.4 μU mL-1（3σ/N 时），回收率为 98.1-101.2%。此外，该免疫传感器还被用于检测真实血清样本，结果与酶联免疫吸附试验（ELISA）的结果一致，表明所提出的免疫传感器是临床诊断应用的理想平台。

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