Asparaginase and isoaspartyl peptidase 1 RNA interference suppresses the growth of nasopharyngeal carcinoma cells.

IF 2.8 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM Discover. Oncology Pub Date : 2024-11-09 DOI:10.1007/s12672-024-01228-1
Bo Feng, Yingying Pei, Weiwei Zhang, Qi Zheng, Yan Zhou
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Abstract

Nasopharyngeal carcinoma (NPC) is one of the common malignant tumors, and its pathogenesis has not been fully clarified. This study aims to explore the impact of RNA interference on the growth and invasion of NPC cells. Asparaginase and isoaspartyl peptidase 1 (ASRGL1)-short hairpin(sh) RNA expressing lentivirus was used to investigate the effect of ASRGL1 knockdown on NPC cells (C666-1 and SUN-1). The target shASRGL1 gene was determined by mRNA and protein expression in nasopharyngeal carcinoma cells; nasopharyngeal carcinoma cell proliferation viability, migration, invasion, apoptosis, ATP levels, and oxidative stress were examined. The results found that ASRGL1 was found to be highly expressed in NPC tissues and cell lines. shASRGL1 exhibited a high gene expression knockdown efficiency, downregulated the ASRGL1 protein expression in the nasopharyngeal carcinoma cells, suppressed proliferation viability of transfected nasopharyngeal carcinoma cells, inhibited their migration and invasion and ATP levels, promoted nasopharyngeal carcinoma cell apoptosis, ROS, and ferroptosis. shASRGL1 plays a role in protecting against NPC cell growth and invasion.

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天冬酰胺酶和异天冬氨酰肽酶 1 RNA 干扰可抑制鼻咽癌细胞的生长。
鼻咽癌是常见的恶性肿瘤之一,其发病机制尚未完全明确。本研究旨在探讨 RNA 干扰对鼻咽癌细胞生长和侵袭的影响。研究使用表达短发夹(sh)RNA的天冬酰胺酶和异天冬氨酰肽酶1(ASRGL1)慢病毒来研究ASRGL1敲除对鼻咽癌细胞(C666-1和SUN-1)的影响。通过鼻咽癌细胞的 mRNA 和蛋白表达确定了 shASRGL1 的靶基因,并检测了鼻咽癌细胞的增殖活力、迁移、侵袭、凋亡、ATP 水平和氧化应激。结果发现 ASRGL1 在鼻咽癌组织和细胞系中高表达。shASRGL1 具有较高的基因表达敲除效率,能下调鼻咽癌细胞中 ASRGL1 蛋白的表达,抑制转染鼻咽癌细胞的增殖活力,抑制其迁移、侵袭和 ATP 水平,促进鼻咽癌细胞凋亡、ROS 和铁变态反应。
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来源期刊
Discover. Oncology
Discover. Oncology Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
2.40
自引率
9.10%
发文量
122
审稿时长
5 weeks
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