Unlocking the potential of dried blood spot sampling for SARS-CoV-2 antibody detection: A pathway to efficient pandemic surveillance

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES Diagnostic microbiology and infectious disease Pub Date : 2024-11-04 DOI:10.1016/j.diagmicrobio.2024.116581

Ahmad M. Alharbi

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Abstract

Summary

The current SARS-CoV-2 pandemic has exerted unprecedented pressure on global healthcare systems, with an urgent demand for widespread testing to monitor virus spread and contribute to disease control.

Aim

This study aimed to validate the use of Dried Blood Spot (DBS) sampling for analyzing the immune response to SARS-CoV-2 infection, employing an accessible enzyme-linked immunosorbent assay (ELISA) for detecting IgG antibodies.

Methodology

Traditional venous blood sampling presents several challenges, including discomfort to patients, risk of healthcare provider exposure, and the need for specialized equipment, which can limit mass testing capabilities. Our research involved the comparison of DBS and venous blood samples from 170 individuals, all of whom had PCR-confirmed SARS-CoV-2 infection, to assess the efficacy of DBS for seroprevalence studies and vaccine response evaluation.

Results

Our results confirmed that DBS sampling could detect SARS-CoV-2 IgG antibodies with 100 % accuracy, showcasing perfect agreement (Cohen's k value of 1.00) between DBS and venous serum samples in detecting the viral antibodies. However, the analysis indicated no significant correlation between the antibody levels in the two sampling methods.

Conclusion

These results suggest that DBS is a viable, rapid alternative for serological studies, offering a solution to current testing limitations and enhancing our public health approach to combating future pandemics.

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挖掘干血斑采样在检测 SARS-CoV-2 抗体方面的潜力：实现高效大流行监测的途径。

当前的 SARS-CoV-2 大流行给全球医疗保健系统带来了前所未有的压力，迫切需要进行广泛的检测，以监测病毒传播并促进疾病控制：方法：传统的静脉采血方法面临着一些挑战，包括患者不适、医护人员暴露的风险，以及需要专用设备，这可能会限制大规模检测能力。我们的研究比较了 DBS 和静脉血样本，这些样本来自 170 人，均为 PCR 确认的 SARS-CoV-2 感染者，以评估 DBS 在血清流行率研究和疫苗反应评估中的有效性：结果：我们的研究结果证实，DBS 抽样检测 SARS-CoV-2 IgG 抗体的准确率为 100%，DBS 和静脉血清样本在检测病毒抗体方面完全一致（Cohen's k 值为 1.00）。然而，分析表明两种采样方法的抗体水平之间没有明显的相关性：这些结果表明，DBS 是血清学研究的一种可行、快速的替代方法，为解决当前检测的局限性提供了一种解决方案，并增强了我们应对未来流行病的公共卫生方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.