Description of Olsenella absiana sp. nov., an anaerobic bacterium isolated from pig faeces.

IF 2 3区生物学 Q4 MICROBIOLOGY International journal of systematic and evolutionary microbiology Pub Date : 2024-11-01 DOI:10.1099/ijsem.0.006579

Jayoung Paek, Lu Bai, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang

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Abstract

A short-rod-shaped, obligate anaerobic, Gram-positive bacterium isolated from the faeces of a pig was designated as the strain YH-ols2223^T. Analysis of the 16S rRNA gene sequence revealed that the isolate was most closely related to Olsenella urininfantis KCTC 25755^T with 97.7% similarity. The multi-locus sequence tree revealed that the isolate formed a sub-cluster within the genus Olsenella. The average nucleotide identity and the digital DNA-DNA hybridization values for the isolate and the most closely related strains were 74.9-76.6% and 20.8-22.5%, respectively. The end products of the fermentation were lactic acid and acetic acid. The main fatty acids identified were C_18 : 0 DMA, C_18 : 1 ω9c DMA and C_18 : 1 ω9c. The cell wall contained the meso-diaminopimelic acid-based peptidoglycan. Given the chemotaxonomic, phenotypic and phylogenetic properties, YH-ols2223^T (= KCTC 25800^T = NBRC 116680^T) represents a novel taxon. The name Olsenella absiana sp. nov. was proposed.

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描述从猪粪便中分离出的厌氧细菌 Olsenella absiana sp.

从猪粪便中分离出的一种短杆状、厌氧、革兰氏阳性细菌被命名为 YH-ols2223T 菌株。16S rRNA 基因序列分析表明，该分离菌与 Olsenella urininfantis KCTC 25755T 的亲缘关系最密切，相似度高达 97.7%。多焦点序列树显示，该分离物在奥尔森氏菌属中形成了一个亚群。该分离株与最密切相关菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 74.9%-76.6% 和 20.8%-22.5%。发酵的最终产物是乳酸和乙酸。鉴定出的主要脂肪酸为 C18 ：0 DMA、C18 ：1 ω9c DMA 和 C18 ：1 ω9c。细胞壁含有以中二氨基丙二酸为基础的肽聚糖。鉴于其化学分类学、表型和系统发育特性，YH-ols2223T（= KCTC 25800T = NBRC 116680T）代表了一个新的类群。拟命名为 Olsenella absiana sp.

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.