Quality Assessment of Corydalis saxicola Bunting Using Quantitative Analysis of Multi-Components by Single Marker and Fingerprint Analysis

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL Journal of separation science Pub Date : 2024-11-14 DOI:10.1002/jssc.70028

Yuanle Shen, Xu Yang, Hongli Feng, Huini Ruan, Yuan Zhou, Zihong Li, Jilang Li, Peng Zhang, Kaitong Li, Honglan Wang, Shen Liu, Xiaoxiu Shi, Jielan Huang, Jinyan Xian, Qiujie Chen, Zhenjie Liu, Jianfang Feng

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Abstract

Yan Huanglian is the whole grass and rhizome of Corydalis saxicola Bunting (CSB) of the Papaveraceae family. CSB has various pharmacological effects and is a promising ethnopharmaceutical. The chromatographic fingerprint of 16 batches of CSB samples was established in this study. Quantitative analysis of multi-components by single marker and the external standard method (ESM) was used to quantitatively assess seven constituents of CSB. Because of its high stability and availability, dehydrocavidine was used as a reference to determine the relative correction factors (RCFs) of columbamine, epiberberine, coptisine hydrochloride, palmatine chloride, berberine hydrochloride, and chelerythrine based on high-performance liquid chromatography. The calculated quantitative analysis of multi-components by single marker values is consistent with those obtained from the ESM, and the repeatability of the RCFs was demonstrated. For the first time, the quantitative analysis of multi-components by the single marker method has been established for the simultaneous determination of seven components, and it is demonstrated to be feasible and accurate for the quality evaluation of CSB combined with chromatographic fingerprint analysis. The combination of these methods could be used as a reference for quality control in Chinese medicine.

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利用单一标记和指纹分析法对多种成分进行定量分析，评估 Corydalis saxicola Bunting 的质量。

炎黄莲是罂粟科植物堇菜（Corydalis saxicola Bunting，CSB）的全草和根茎。CSB具有多种药理作用，是一种很有前途的民族药。本研究建立了 16 批 CSB 样品的色谱指纹图谱。采用单一标记和外标法（ESM）对多成分进行定量分析，定量评估了 CSB 的七种成分。由于脱氢卡维丁具有高稳定性和易得性，因此以其为参照物，通过高效液相色谱法确定了可乐定、表小檗碱、盐酸黄连碱、氯化巴马汀、盐酸小檗碱和诃子碱的相对校正系数（RCF）。通过单一标记值计算出的多成分定量分析结果与通过 ESM 得出的结果一致，并证明了 RCF 的可重复性。首次建立了同时测定七种成分的单标记多成分定量分析方法，并证明该方法与色谱指纹图谱分析相结合用于 CSB 质量评价是可行和准确的。这些方法的组合可作为中药质量控制的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.