Identifying putative allergens from Cenostigma pluviosum pollen using proteomic bioinformatics.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Reports Pub Date : 2024-11-14 DOI:10.1007/s11033-024-10079-6

Jaime Olbrich Neto, Bruna Cavecci-Mendonça, Sandra Regina Leite Rosa Olbrich, Francielle Ramalho Rocha, Eloisa Fornaro Clemente, Bruno Cesar Rossini, Lucilene Delazari Dos Santos

{"title":"Identifying putative allergens from Cenostigma pluviosum pollen using proteomic bioinformatics.","authors":"Jaime Olbrich Neto, Bruna Cavecci-Mendonça, Sandra Regina Leite Rosa Olbrich, Francielle Ramalho Rocha, Eloisa Fornaro Clemente, Bruno Cesar Rossini, Lucilene Delazari Dos Santos","doi":"10.1007/s11033-024-10079-6","DOIUrl":null,"url":null,"abstract":"Background: Routinely, signs and symptoms of pollinosis worsen during the flowering period of the Cenostigma pluviosum var. peltophoroides (Sibipiruna) trees. This study aimed to determine whether Sibipiruna pollen contains allergenic proteins.Methods and results: The pollen from mature Sibipiruna anthers was morphologically characterized using optical, scanning electron and emission microscopy. Additionally, biochemical characterization was conducted through Shotgun Label-Free Proteomic Analysis. Three hundred and forty-nine proteins were identified using the UNIPROT database and thirty-six were confirmed as homologous allergenic proteins in the Allergome database.Conclusions: At the first time, Sibipiruna pollen allergenic proteins have been described and this study provides a deeper understanding of the allergenic proteins present in this pollen. These preliminary findings can be useful to assist in the development of targeted extracts for the study of sensitization and potential immunotherapy in the future.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"51 1","pages":"1155"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Biology Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11033-024-10079-6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Routinely, signs and symptoms of pollinosis worsen during the flowering period of the Cenostigma pluviosum var. peltophoroides (Sibipiruna) trees. This study aimed to determine whether Sibipiruna pollen contains allergenic proteins.

Methods and results: The pollen from mature Sibipiruna anthers was morphologically characterized using optical, scanning electron and emission microscopy. Additionally, biochemical characterization was conducted through Shotgun Label-Free Proteomic Analysis. Three hundred and forty-nine proteins were identified using the UNIPROT database and thirty-six were confirmed as homologous allergenic proteins in the Allergome database.

Conclusions: At the first time, Sibipiruna pollen allergenic proteins have been described and this study provides a deeper understanding of the allergenic proteins present in this pollen. These preliminary findings can be useful to assist in the development of targeted extracts for the study of sensitization and potential immunotherapy in the future.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

利用蛋白质组生物信息学鉴定 Cenostigma pluviosum 花粉中的潜在过敏原。

背景：常规情况下，花粉症的症状和体征在Cenostigma pluviosum var.本研究旨在确定西比鲁纳花粉是否含有致敏蛋白质：使用光学显微镜、扫描电子显微镜和发射显微镜对成熟花药的花粉进行了形态学鉴定。此外，还通过无标记蛋白质组分析（Shotgun Label-Free Proteomic Analysis）进行了生化鉴定。利用 UNIPROT 数据库鉴定了 349 种蛋白质，并在 Allergome 数据库中确认了 36 种同源过敏原蛋白质：本研究首次描述了 Sibipiruna 花粉中的过敏原蛋白，并加深了对该花粉中过敏原蛋白的了解。这些初步发现有助于开发有针对性的提取物，用于研究过敏性和未来潜在的免疫疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.