[Euphorbia helioscopia inhibits proliferation, invasion, and migration and promotes apoptosis of non-small cell lung cancer cells].

Q3 Medicine 南方医科大学学报杂志 Pub Date : 2024-10-20 DOI:10.12122/j.issn.1673-4254.2024.10.10

X Liu, Y Yang, W Liu, Z Zhang, X Zhou, W Xie, L Shen, M Zhang, X Li, J Zang, S Li

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Abstract

Objective: To investigate the effect of Euphorbia helioscopia on biological behaviors of non-small cell lung cancer (NSCLC) cells.

Methods: NSCLC cell lines PC-9 and A549 treated with different concentrations of Euphorbia helioscopia preparations were examined for changes in proliferation, apoptosis, invasion and migration using CCK-8 assay, colony formation assay, flow cytometry, wound healing assay and Transwell assay. Western blotting was performed to detect the changes in protein expressions of Bax, Bcl-2, E-cadherin, vimentin, MMP2, and MMP9 in the treated cells. PC-9 cells were injected subcutaneously into BALB/C nude mice to establish a nude mouse subcutaneous tumor model. According to the growth of subcutaneous tumors, mice were randomly divided into control group: gavaged daily with saline; Euphorbia helioscopia-treated group: gavaged daily with Euphorbia helioscopia 65 mg/mL, and Euphorbia helioscopia granules were dissolved in saline; cisplatin-treated group: injected intraperitoneally with cisplatin 4 mg/kg every 5 days, 6 mice per group. The subcutaneous tumor volume and mass changes of mice were measured, and the toxic effects of Euphorbia helioscopia on heart, liver, spleen, lung and kidney as well as the therapeutic effects of Euphorbia helioscopia were observed in the mice bearing tumor.

Results: Euphorbia helioscopia granules concentration-dependently inhibited the proliferation and survival of PC-9 and A549 cells, significantly promoted cell apoptosis, suppressed invasion and migration abilities of the cells, up-regulated the expression levels of E-cadherin and Bax, and down-regulated the expressions of Bcl-2, vimentin, MMP2, and MMP9. In the tumor-bearing mice, treatment with Euphorbia helioscopia significantly inhibited tumor growth without producing obvious toxicity in the vital organs.

Conclusion: Euphorbia helioscopia can inhibit proliferation, invasion, and migration and induces apoptosis of NSCLC cells in vitro.

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[大戟提取物能抑制非小细胞肺癌细胞的增殖、侵袭和迁移，并促进其凋亡】。］

目的研究大戟提取物对非小细胞肺癌（NSCLC）细胞生物学行为的影响：方法：用不同浓度的大戟提取物处理 NSCLC 细胞株 PC-9 和 A549，采用 CCK-8 试验、菌落形成试验、流式细胞术、伤口愈合试验和 Transwell 试验检测细胞增殖、凋亡、侵袭和迁移的变化。用 Western 印迹法检测处理过的细胞中 Bax、Bcl-2、E-cadherin、vimentin、MMP2 和 MMP9 蛋白表达的变化。将 PC-9 细胞皮下注射到 BALB/C 裸鼠体内，建立裸鼠皮下肿瘤模型。根据小鼠皮下肿瘤的生长情况，将其随机分为对照组：每天用生理盐水灌胃；大戟治疗组：每天用大戟65 mg/mL灌胃，大戟颗粒溶于生理盐水；顺铂治疗组：腹腔注射顺铂4 mg/kg，每5天一次，每组6只。测定小鼠皮下肿瘤体积和质量变化，观察大戟提取物对小鼠心、肝、脾、肺、肾的毒性作用以及大戟提取物对肿瘤的治疗作用：结果：大戟提取物颗粒浓度依赖性抑制PC-9和A549细胞的增殖和存活，显著促进细胞凋亡，抑制细胞的侵袭和迁移能力，上调E-cadherin和Bax的表达水平，下调Bcl-2、波形蛋白、MMP2和MMP9的表达。在肿瘤小鼠体内，大戟提取物能显著抑制肿瘤生长，且不会对重要器官产生明显毒性：结论：大戟能在体外抑制 NSCLC 细胞的增殖、侵袭和迁移，并诱导其凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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