LINC00470 promotes malignant progression of testicular germ cell tumors.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Reports Pub Date : 2024-11-14 DOI:10.1007/s11033-024-10083-w

Zhizhong Liu, Shanshan Lv, Zailong Qin, Jinhui Shu, Fang Zhu, Yanwei Luo, Liqing Fan, Mengqian Chen, Hao Bo, Lvjun Liu

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Abstract

Background: Testicular germ cell tumor (TGCT) is a common malignant tumor in adolescents. Now, many long non-coding RNAs (LncRNAs) have been found to have an important function in TGCT. LINC00470 is specifically and highly expressed in TGCT, however, there is still no definite information concerning its role and underlying mechanism in TGCT. The purpose of this research was to look into the involvement of LINC00470 in TGCT and its intrinsic mechanism.

Methods and results: UCSC and GEPIA2 databases were used to analyze the expression of LINC00470, and the BEST website was used to perform GSEA enrichment analysis, immune infiltration analysis, and drug susceptibility analysis. SiRNA transfection was used to silence LINC00470 in TCAM-2 and NCCIT cells. Clone formation and Transwell assays were performed in TGCT cells to confirm the effects of LINC00470 on clone formation, migration, and invasion. Western Blot was performed to determine the expression of proteins related to the EMT and AKT signaling pathways. LINC00470 was specifically highly expressed in TGCT, and played a role in promoting tumor cell clone formation and cell metastasis by affecting the TGF-β and PI3K-AKT-mTOR signaling pathways to regulate the epithelial-mesenchymal transition (EMT) process; LINC00470 may also play a pro-tumor role by negatively regulating immune infiltration; in addition, the expression of LINC00470 was negatively correlated with the chemosensitivity of cisplatin in TGCT patients.

Conclusions: LINC00470 may play a significant role in the etiology and metastasis of TGCT through EMT and AKT-mediated signaling pathways.

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LINC00470会促进睾丸生殖细胞肿瘤的恶性发展。

背景：睾丸生殖细胞瘤（TGCT）是青少年常见的恶性肿瘤：睾丸生殖细胞瘤（TGCT）是青少年常见的恶性肿瘤。目前，许多长非编码 RNA（LncRNA）被发现在 TGCT 中具有重要功能。LINC00470在TGCT中特异性高表达，但关于它在TGCT中的作用和内在机制仍无明确信息。本研究的目的是探讨 LINC00470 在 TGCT 中的参与及其内在机制：利用 UCSC 和 GEPIA2 数据库分析 LINC00470 的表达，并利用 BEST 网站进行 GSEA 富集分析、免疫浸润分析和药物敏感性分析。SiRNA转染用于沉默TCAM-2和NCCIT细胞中的LINC00470。在 TGCT 细胞中进行克隆形成和 Transwell 试验，以确认 LINC00470 对克隆形成、迁移和侵袭的影响。通过 Western Blot 检测与 EMT 和 AKT 信号通路相关的蛋白表达。LINC00470在TGCT中特异性高表达，并通过影响TGF-β和PI3K-AKT-mTOR信号通路来调控上皮-间质转化（EMT）过程，从而在促进肿瘤细胞克隆形成和细胞转移方面发挥作用；LINC00470还可能通过负向调节免疫浸润而起到促瘤作用；此外，LINC00470的表达与TGCT患者对顺铂的化疗敏感性呈负相关。结论LINC00470可能通过EMT和AKT介导的信号通路在TGCT的病因学和转移中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.