Electroacupuncture alleviated chronic cerebral hypoperfusion damages via targeting aquaporin 4 to prevent amyloid-beta accumulation.

IF 1.6 4区医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-11-12 DOI:10.1097/WNR.0000000000002114

Ying Zhang, Lei Wu, Kelin He, Yingying Cheng, Lu Li, Dexiong Han

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Abstract

The present study aimed to investigate the therapeutic effects of electroacupuncture (EA) on chronic cerebral hypoperfusion (CCH). We first applied the Morris water maze approach to determine the effects of EA and TGN-020 [an inhibitor of aquaporin 4 (AQP4)] on the learning and memory ability of CCH rats. The hematoxylin and eosin, and Nissl staining were further used to investigate the effects of EA and TGN-020 on the neuropathological changes of the dentate gyrus. Next, the ELISA kits were adopted to determine the effects of EA and TGN-020 on the content of amyloid-beta (Aβ) in the cerebrospinal fluid of CCH rats. Finally, we respectively employed technologies of immunohistochemical staining, quantitative real-time PCR, and Western blot to further explore the effects of EA and TGN-020 on the mRNA expression level of amyloid precursor protein (APP) and AQP4 as well as the protein expression level of Aβ1-42 and AQP4 in the dentate gyrus of CCH rats. Our results indicated that EA not only enhanced the learning and memory abilities of CCH rats but also improved the neuropathological damages of CCH rats by upregulating the mRNA and protein expression level of AQP4 to reduce the accumulation of Aβ, especially for the reduction of the mRNA expression level of APP and the protein expression level of Aβ1-42, but TGN-020 effectively reversed the therapeutic effects mentioned above of EA. In summary, we proved that EA, as the activator of AQP4, prevents the accumulation of Aβ during the treatment of CCH.

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电针通过靶向水蒸发蛋白4防止淀粉样蛋白-β积聚减轻慢性脑灌注不足的损害

本研究旨在探讨电针（EA）对慢性脑灌注不足（CCH）的治疗作用。我们首先应用 Morris 水迷宫法测定 EA 和 TGN-020 [一种水蒸气素 4（AQP4）抑制剂] 对慢性脑缺血大鼠学习和记忆能力的影响。苏木精、伊红和 Nissl 染色法进一步研究了 EA 和 TGN-020 对齿状回神经病理学变化的影响。接着，我们采用ELISA试剂盒测定了EA和TGN-020对CCH大鼠脑脊液中淀粉样β（Aβ）含量的影响。最后，我们分别采用免疫组化染色、定量实时PCR和Western blot技术进一步探讨了EA和TGN-020对CCH大鼠齿状回中淀粉样前体蛋白（APP）和AQP4 mRNA表达水平以及Aβ1-42和AQP4蛋白表达水平的影响。我们的研究结果表明，EA不仅能提高CCH大鼠的学习和记忆能力，还能通过上调AQP4的mRNA和蛋白表达水平来减少Aβ的积累，从而改善CCH大鼠的神经病理损伤，尤其是能降低APP的mRNA表达水平和Aβ1-42的蛋白表达水平，但TGN-020能有效逆转EA的上述治疗作用。综上所述，我们证明了EA作为AQP4的激活剂，在治疗CCH过程中可防止Aβ的蓄积。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.