Evaluation of the Effect of Growth Factors on Chlorophylls a and b Production from Microalgae.

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Jove-Journal of Visualized Experiments Pub Date : 2024-10-25 DOI:10.3791/67208
Frizek Nathaniel Morales-Rivera, Sofia Torres-Momber, Julio César Jacuinde-Ruíz, Juan Cristóbal Camacho-Arriaga, Juan Carlos González-Hernández
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Abstract

Microalgae contain two main groups of pigments: chlorophylls and carotenoids. Chlorophyll is a green pigment that absorbs light energy and transforms it into chemical energy to facilitate the synthesis of organic compounds. This pigment serves as a valuable primary source for biotechnological input products in the food, pharmaceutical, and cosmetic industries due to its high antioxidant properties and coloring capabilities. The objective of this research was to evaluate the effect of growth factors (CO2 concentration, light color, and light intensity) through a Taguchi L4 experimental design on cell growth and the cellular content of chlorophyll a and b in Chlorella sorokiniana, followed by validation of the method using Haematococcus pluvialis microalgae as an additional study model. Cell growth was quantified using the optical density spectrophotometric technique at a wavelength of 550 nm. For the quantification of chlorophylls, a cell extract was obtained using a 90% pure acetone solution, and subsequently, the concentrations of chlorophylls a and b were quantified using spectrophotometric techniques at wavelengths of 647 nm and 664 nm, according to the method described by Jeffrey and Humphrey. The experimental results indicated that controlling conditions of low CO2 addition, purple light, and low light intensity increases both cell growth and the concentration of chlorophylls a and b within the cells. The implementation of this chlorophyll quantification method allows for quick, simple, and precise determination of chlorophyll content, as the wavelengths used are at the absorbance peaks of both types of chlorophylls, making this technique easily reproducible for any microalgae under study.

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评估生长因子对微藻叶绿素 a 和 b 产量的影响
微藻类含有两大类色素:叶绿素和类胡萝卜素。叶绿素是一种绿色色素,能吸收光能并将其转化为化学能,促进有机化合物的合成。这种色素具有高抗氧化性和着色能力,是食品、制药和化妆品行业中生物技术投入产品的重要主要来源。本研究的目的是通过田口 L4 实验设计,评估生长因素(二氧化碳浓度、光色和光照强度)对小球藻细胞生长以及叶绿素 a 和叶绿素 b 细胞含量的影响。细胞生长的定量采用波长为 550 纳米的光密度分光光度法。在叶绿素的定量方面,使用纯度为 90% 的丙酮溶液提取细胞萃取液,然后根据 Jeffrey 和 Humphrey 描述的方法,使用波长为 647 nm 和 664 nm 的分光光度技术对叶绿素 a 和 b 的浓度进行定量。实验结果表明,控制低二氧化碳添加量、紫光和低光照强度的条件既能增加细胞的生长,又能提高细胞内叶绿素 a 和 b 的浓度。采用这种叶绿素定量方法可以快速、简便、精确地测定叶绿素含量,因为所使用的波长处于两种叶绿素的吸光峰值,因此这种技术对于研究中的任何微藻类都很容易重复。
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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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