Association between the G/G genotype of the lncRNA MEG3 rs7158663 polymorphism and proliferative diabetic retinopathy.

IF 1.6 4区医学 Q4 ENDOCRINOLOGY & METABOLISM Archives of Endocrinology Metabolism Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI:10.20945/2359-4292-2024-0024

Leticia de Almeida Brondani, Isabele Dandolini, Eliandra Girardi, Luís Henrique Canani, Daisy Crispim, Cristine Dieter

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Abstract

Objective: To investigate the association between the long noncoding RNAs (lncRNAs) maternally expressed gene 3 (MEG3) rs7158663 polymorphism and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (T2DM).

Subjects and methods: The study included 628 patients with T2DM and DR ("case group," including 283 with proliferative DR [PDR] and 345 with nonproliferative DR [NPDR]), and 381 patients with T2DM but no DR ("control group"). The diagnosis of DR was established using indirect ophthalmoscopy. The rs7158663 A/G polymorphism was genotyped using real-time polymerase chain reaction (PCR) with TaqMan probes.

Results: Patients with DR, compared with those without DR, had lower frequencies of both the G/G genotype (17.5% and 23.6%, respectively, p = 0.044) and the G allele (p = 0.017). When only patients with PDR were compared with controls, the G/G genotype was associated with increased protection against PDR after adjustment (odds ratio 0.551, 95% confidence interval 0.314-0.966, p = 0.038). This association also remained in the dominant (p = 0.036) and additive (p = 0.031) genetic models.

Conclusion: This study reveals, for the first time, that the G/G genotype of the lncRNA MEG3 rs7158663 single-nucleotide polymorphism is associated with a protective effect against advanced-stage DR in patients with T2DM. Additional studies are warranted to validate this finding.

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lncRNA MEG3 rs7158663 多态性的 G/G 基因型与增殖性糖尿病视网膜病变之间的关系。

目的探讨长非编码 RNA（lncRNA）母体表达基因 3（MEG3）rs7158663 多态性与 2 型糖尿病（T2DM）患者糖尿病视网膜病变（DR）之间的关联：研究对象包括 628 名患有 T2DM 和 DR 的患者（"病例组"，包括 283 名增殖性 DR [PDR] 和 345 名非增殖性 DR [NPDR]），以及 381 名患有 T2DM 但没有 DR 的患者（"对照组"）。DR的诊断是通过间接眼底镜检查确定的。使用实时聚合酶链反应（PCR）和 TaqMan 探针对 rs7158663 A/G 多态性进行基因分型：结果：与非 DR 患者相比，DR 患者的 G/G 基因型（分别为 17.5%和 23.6%，p = 0.044）和 G 等位基因（p = 0.017）频率均较低。当仅将 PDR 患者与对照组进行比较时，经调整后，G/G 基因型与 PDR 防护能力增强相关（几率比 0.551，95% 置信区间 0.314-0.966，p = 0.038）。这种关联在显性（p = 0.036）和加性（p = 0.031）遗传模型中也保持不变：本研究首次揭示了 lncRNA MEG3 rs7158663 单核苷酸多态性的 G/G 基因型与 T2DM 患者晚期 DR 的保护作用相关。为了验证这一发现，还需要进行更多的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Endocrinology Metabolism Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

2.90

自引率

5.90%

发文量

107

审稿时长

7 weeks

期刊介绍： The Archives of Endocrinology and Metabolism - AE&M – is the official journal of the Brazilian Society of Endocrinology and Metabolism - SBEM, which is affiliated with the Brazilian Medical Association. Edited since 1951, the AE&M aims at publishing articles on scientific themes in the basic translational and clinical area of Endocrinology and Metabolism. The printed version AE&M is published in 6 issues/year. The full electronic issue is open access in the SciELO - Scientific Electronic Library Online e at the AE&M site: www.aem-sbem.com. From volume 59 on, the name was changed to Archives of Endocrinology and Metabolism, and it became mandatory for manuscripts to be submitted in English for the online issue. However, for the printed issue it is still optional for the articles to be sent in English or Portuguese. The journal is published six times a year, with one issue every two months.