Thermosensitive Injectable Dual Drug-Loaded Chitosan-Based Hydrogels for Treating Bacterial Endometritis.

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-12-09 Epub Date: 2024-11-15 DOI:10.1021/acsbiomaterials.4c01729

Xin Wang, Zheng Wei, Zuoyao Wu, Yanping Li, Chenjiao Miao, Zheng Cao

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Abstract

Endometritis, a prevalent obstetric condition primarily caused by Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), significantly threatens the reproductive performance of female animals. In this study, thermosensitive injectable chitosan (CS)/β-glycerophosphate (β-GP) hydrogels loaded with berberine (BBR) and carvacrol (CAR) were prepared for endometritis treatment. In vitro, BBR/CAR-CS/β-GP hydrogels exhibited rapid gelation within 5 min at 37 °C, excellent injectability, and more than 90% degradation within 30 days under enzymatic action. The dual drug-loaded system also exhibited controlled release of BBR and CAR and demonstrated the antimicrobial activity against E. coli and S. aureus. In vivo, uterine injection of BBR/CAR-CS/β-GP hydrogels alleviated infection-induced injuries and reduced the bacterial load in infected uterine tissues. In summary, these findings highlight the potential of BBR/CAR-CS/β-GP hydrogels as innovative carriers for drug delivery targeting endometritis.

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用于治疗细菌性子宫内膜炎的热敏注射双药壳聚糖水凝胶

子宫内膜炎是一种产科常见病，主要由大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）引起，严重威胁雌性动物的生殖能力。本研究制备了载有小檗碱（BBR）和香芹酚（CAR）的热敏注射用壳聚糖（CS）/β-甘油磷酸酯（β-GP）水凝胶，用于治疗子宫内膜炎。在体外，BBR/CAR-CS/β-GP 水凝胶在 37 °C、5 分钟内迅速凝胶化，具有良好的注射性，在酶作用下 30 天内降解率超过 90%。该双重载药系统还能控制 BBR 和 CAR 的释放，并对大肠杆菌和金黄色葡萄球菌具有抗菌活性。在体内，子宫注射 BBR/CAR-CS/β-GP 水凝胶可减轻感染引起的损伤，并减少受感染子宫组织中的细菌量。总之，这些研究结果凸显了 BBR/CAR-CS/β-GP 水凝胶作为创新载体用于靶向子宫内膜炎给药的潜力。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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