A study of clinical and bacteriological characteristics of Klebsiella pneumoniae isolates in Birjand, South-East Iran: Hypervirulent phenotype, biofilm formation, virulence factors, and antibiotic resistance

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES Diagnostic microbiology and infectious disease Pub Date : 2024-11-04 DOI:10.1016/j.diagmicrobio.2024.116595

Ebrahim Shafaie , Mohammad Hosein Mahdizadeh , Masoumeh Amirian , Parvin Askari , Masoud Yousefi

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Abstract

This study assessed antimicrobial susceptibility, biofilm formation, hypervirulence traits, and virulence-related genes in K. pneumoniae. 119 isolates underwent antibiotic susceptibility testing using the Kirby-Bauer method, biofilm assessment through Congo red agar and Microtiter plate assays, PCR for various genes, and the String test for hypermucoviscosity. Clinical characteristics and virulence factors of hypervirulent (hvKP) were compared to classic K. pneumoniae (cKP) strains. Among the isolates, 77.3% were multi-drug resistant (MDR), and 83.2% showed biofilm-forming ability, with a higher MDR incidence in biofilm producers. A significant correlation was found between biofilm formation and the presence of the bla_CTX-M15 gene. Genes luxS, mrkA, pgaA, and wzm were significantly related to biofilm production. Three K. pneumoniae (2.5%) were identified as hvKP, with higher prevalence of capsular serotypes K1/K2 and virulence-related genes iuc, rmpA, and rmpA2 than cKP. The study underscores the importance of surveillance and stewardship in combating MDR, biofilm-forming, highly virulent K. pneumoniae.

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伊朗东南部比尔詹德肺炎克雷伯氏菌分离物的临床和细菌学特征研究：高病毒表型、生物膜形成、毒力因子和抗生素耐药性。

本研究对肺炎克雷伯菌的抗菌药敏感性、生物膜形成、高病毒性特征和毒力相关基因进行了评估。对 119 个分离株采用柯比鲍尔法进行了抗生素敏感性检测，通过刚果红琼脂和微孔板检测法进行了生物膜评估，对各种基因进行了 PCR 检测，并对高黏滞性进行了 String 检测。将高病毒性（hvKP）菌株的临床特征和毒力因素与传统肺炎克氏菌（cKP）菌株进行了比较。在分离出的菌株中，77.3%具有多重耐药性（MDR），83.2%具有生物膜形成能力，生物膜产生者的MDR发生率更高。研究发现，生物膜的形成与 blaCTX-M15 基因的存在有明显的相关性。基因luxS、mrkA、pgaA和wzm与生物膜的形成有显著相关性。与 cKP 相比，有三种肺炎克氏菌（2.5%）被鉴定为 hvKP，其菌盖血清型 K1/K2 和毒力相关基因 iuc、rmpA 和 rmpA2 的流行率更高。这项研究强调了监测和管理在抗击 MDR、生物膜形成型高致病性肺炎克菌中的重要性。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.