Approaches and findings in histological and micromorphological research on Rhizostomeae.

Abstract

The substantial development of microscopic techniques and histological examination methods during the past five decades allowed for many new insights into the histology and microanatomy of Rhizostomeae. The present review focuses on new findings about histologically important structures: nerves, senses, muscles, gonads, zooxanthellae and nematocysts. Different ontogenetic stages of rhizostome species were included in the literature research, supplemented with the authors' unpublished data and figures. The overview of the research results reveals that the application of chemo- and immunohistochemical techniques have provided deeper insights into neuronal and sensory structures and their interconnections. Modern microscopic methods led to new findings on the histological gonadal organization and details of the processes of gametogenesis, fertilization, cleavage, gastrulation, and brooding. Advanced optical methods also allowed for a better understanding of Rhizostomeae-zooxanthellae associations and the morphology and function of nematocysts. Improvements in molecular biology allowed for more precise identification of zooxanthellae associated with rhizostome species. Although there has been significant progress in all of the research subjects covered here, we identify several knowledge gaps and conclude with some recommendations for future research.