The Beneficial Role of Low PVP Concentration on Sperm Apoptotic Gene Expression, Embryo Morphokinetics Status, and Clinical ICSI Outcomes

IF 2.1 4区 医学 Q3 ANDROLOGY Andrologia Pub Date : 2024-11-08 DOI:10.1155/2024/8803887
Arefeh Akbari Javar, Behrouz Aflatoonian, Ali Reza Talebi, Fatemeh Dehghanpour, Mohammad Ali Khalili, Fateme Montazeri
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Abstract

During the intracytoplasmic sperm injection (ICSI) procedure, 10% polyvinylpyrrolidone (PVP) is commonly used as a selection medium to decrease sperm motility. The study aimed to determine the effect of different concentrations of PVP (5% and 10%) on sperm apoptotic transcripts, sperm DNA fragmentation (SDF), embryo cytokinetic, and clinical characteristics in the ICSI program. In the study, 60 couples with male factor infertility underwent ICSI insemination using either 5% or 10% PVP concentration. Metaphase II (MII) oocytes were divided into two groups, with one group injected with sperm in 5% PVP and the other in 10% PVP. After fertilization, the zygotes were cultured using a time-lapse microscope to assess morphokinetic parameters also, simultaneously with the ICSI procedure, SDF and apoptotic transcript levels were quantified using reverse transcription quantitative polymerase chain reaction (qPCR). The study found that DNA fragmentation was lower in sperm from the 5% PVP group compared to the 10% PVP group. Additionally, the expression levels of B-cell lymphoma (BCL2) and the 70 kilodalton heat shock proteins (HSP70) genes were significantly higher in 5% PVP compared to 10% PVP, while the transcript levels of the Bcl-2-associated X protein (BAX) gene were lower in 5% PVP. The 5% PVP group also demonstrated higher fertilization and high-quality embryo formation rates. Cytokinetic variables were significantly different between the two groups, and the rates of fragmentation, uneven blastomere, reverse cleavage (RC), and embryo arrest were higher in the 10% PVP group. The study concluded that a low concentration of PVP (5%) is a suitable replacement for 10% PVP in ICSI cycles for male factor infertility.

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低 PVP 浓度对精子凋亡基因表达、胚胎形态动力学状态和临床 ICSI 结果的有益作用
在卵胞浆内单精子显微注射(ICSI)过程中,10%的聚乙烯吡咯烷酮(PVP)通常被用作降低精子活力的选择介质。该研究旨在确定不同浓度的PVP(5%和10%)对ICSI程序中精子凋亡转录本、精子DNA碎片(SDF)、胚胎细胞动力学和临床特征的影响。在这项研究中,60对男性因素不育夫妇接受了ICSI人工授精,使用了5%或10%浓度的PVP。将分裂期II(MII)卵母细胞分为两组,一组注入5% PVP浓度的精子,另一组注入10% PVP浓度的精子。受精后,使用延时显微镜对子实体进行培养,以评估形态动力学参数,并在进行 ICSI 过程的同时,使用反转录定量聚合酶链反应(qPCR)对 SDF 和凋亡转录物水平进行量化。研究发现,与 10% PVP 组相比,5% PVP 组精子的 DNA 断裂程度较低。此外,5% PVP 组中 B 细胞淋巴瘤(BCL2)和 70 千道尔顿热休克蛋白(HSP70)基因的表达水平明显高于 10% PVP 组,而 Bcl-2 相关 X 蛋白(BAX)基因的转录水平则低于 5% PVP 组。5% PVP 组的受精率和优质胚胎形成率也更高。细胞运动变量在两组之间存在显著差异,10% PVP 组的破碎率、胚泡不均匀率、反向裂解率(RC)和胚胎停育率较高。研究得出结论,在治疗男性因素不孕症的卵胞浆内单精子显微注射周期中,低浓度 PVP(5%)可替代 10% PVP。
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来源期刊
Andrologia
Andrologia 医学-男科学
CiteScore
5.60
自引率
8.30%
发文量
292
审稿时长
6 months
期刊介绍: Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
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