Syringin ameliorates dextran sulphate colitis via alteration oxidative stress, inflammation NF-κB signalling pathway and gut microbiota.

IF 2.7 4区医学 Q2 PHARMACOLOGY & PHARMACY Basic & Clinical Pharmacology & Toxicology Pub Date : 2024-11-16 DOI:10.1111/bcpt.14105

Juhui Zhao, Qingqing Zhang, Xudong Hao

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Abstract

Background: The objective of the current study was to investigate the potential effects of syringin against dextran sulphate colitis (DSS)-induced ulcerative colitis (UC) in mice.

Material and methods: In vitro study was performed on the RAW 264.7 cells and cytokines and inflammatory level were estimated. The oxidative stress, inflammatory cytokines, apoptosis and inflammatory parameters were estimated. The mRNA expression and faecal samples were estimated in the colon tissue.

Results: Syringin treatment enhanced the body weight, colon length and reduced the disease activity index (DAI), spleen index. Syringin treatment remarkably suppressed the level of nitric oxide (NO), myeloperoxidase (MPO), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) along with alteration of antioxidant parameters. Syringin treatment also altered level of cytokines in the serum and colon tissue; inflammatory parameters viz., platelet-activating factor (PAF), cyclooxygenase-2 (COX-2), prostaglandin (PGE₂), inducible nitric oxide synthetase (iNOS), nuclear factor κ-B (NF-κB); matrix metalloproteinases (MMP) level. Syringin significantly (p < 0.001) enhanced the level of nuclear factor erythroid 2-related factor (Nrf₂) and heme oxygenase-1 (HO-1). Syringin remarkably altered the relative abundance of gut microbiota like Firmicutes, Bacteroidetes, F/B ratio, Verrucomicrobia and Actinobacteria.

Conclusion: Syringin exhibited the protective effect against DSS-induced UC in mice via alteration of NF-κB signalling pathway.

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丁香素通过改变氧化应激、炎症NF-κB信号通路和肠道微生物群来改善硫酸葡聚糖结肠炎

背景：本研究旨在探讨丁香素对硫酸右旋糖酐结肠炎（DSS）诱导的小鼠溃疡性结肠炎（UC）的潜在作用：对 RAW 264.7 细胞进行体外研究，评估细胞因子和炎症水平。对氧化应激、炎症细胞因子、细胞凋亡和炎症参数进行了估计。对结肠组织中的 mRNA 表达和粪便样本进行了估计：结果：鞘氨醇治疗提高了患者的体重和结肠长度，降低了疾病活动指数（DAI）和脾脏指数。鞘氨醇治疗显著抑制了一氧化氮（NO）、髓过氧化物酶（MPO）、细胞间粘附分子-1（ICAM-1）和血管细胞粘附分子-1（VCAM-1）的水平，并改变了抗氧化参数。丁香素还改变了血清和结肠组织中的细胞因子水平；炎症参数，即血小板活化因子（PAF）、环氧化酶-2（COX-2）、前列腺素（PGE2）、诱导型一氧化氮合成酶（iNOS）、核因子κ-B（NF-κB）；基质金属蛋白酶（MMP）水平。紫丁香苷明显（p 2）影响血红素加氧酶-1（HO-1）。人参皂苷明显改变了肠道微生物群的相对丰度，如固着菌、类杆菌、F/B 比率、Verrucomicrobia 和放线菌：结论：Syringin通过改变NF-κB信号通路对DSS诱导的小鼠UC具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Basic & Clinical Pharmacology & Toxicology 医学-毒理学

CiteScore

5.60

自引率

6.50%

发文量

126

审稿时长

1 months

期刊介绍： Basic & Clinical Pharmacology and Toxicology is an independent journal, publishing original scientific research in all fields of toxicology, basic and clinical pharmacology. This includes experimental animal pharmacology and toxicology and molecular (-genetic), biochemical and cellular pharmacology and toxicology. It also includes all aspects of clinical pharmacology: pharmacokinetics, pharmacodynamics, therapeutic drug monitoring, drug/drug interactions, pharmacogenetics/-genomics, pharmacoepidemiology, pharmacovigilance, pharmacoeconomics, randomized controlled clinical trials and rational pharmacotherapy. For all compounds used in the studies, the chemical constitution and composition should be known, also for natural compounds.